Mouse Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit | TRPM4 elisa kit

Mouse Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit

Cat. Num. AAA9716665

• Gene Names: Trpm4; LTRPC4; TRPM4B; LTrpC-4; AW047689; 1110030C19Rik
• Reactivity: Mouse
• Synonyms: Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4); Mouse Transient Receptor Potential Cation Channel Subfamily M Member 4 (TRPM4) ELISA Kit; TRPM4; FLJ20041; TRPM4B; TRPM4 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRPM4. No significant cross-reactivity or interference between Mouse TRPM4 and analogues was observed.
• Sequence Length: 1213

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) Standard Curve.)

Related Product Information for TRPM4 elisa kit

Description: This Mouse Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM4 in samples. An antibody specific for TRPM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRPM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: TRPM4 has 1,040 amino acids and contains 6 putative transmembrane domains. Northern blot analysis detected TRPM4 expression in most adult tissues tested, with highest levels in heart, prostate, and colon. In fetus, TRPM4 expression was most abundant in kidney. There were at least 2 distinct bands detected (6.2 and 4.2 kb), indicating alternative splicing of TRPM4. In addition, a smaller but relatively abundant 2.4-kb transcript was detected in testis.The shorter protein results from alternative splicing, with the third and fourth exons being spliced out and the beginning of the first exon being truncated. As a consequence, the first in-frame methionine lies in the fifth exon, resulting in the deletion of the first 174 amino acids.

NCBI and Uniprot Product Information

• NCBI GI #: 157824093
• NCBI GeneID: 68667
• NCBI Accession #: NP_780339.2
• NCBI GenBank Nucleotide #: NP_780339.2
• UniProt Accession #: Q7TN37
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 4
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily M, member 4
• NCBI Official Symbol: Trpm4
• NCBI Official Synonym Symbols: LTRPC4; TRPM4B; LTrpC-4; AW047689; 1110030C19Rik
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 4
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 4
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: Trpm4
• UniProt Synonym Gene Names: Ltrpc4; LTrpC-4; LTrpC4

Uniprot Description

Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca2+, it is impermeable to it. Mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca2+ overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway (). Essential for the migration but not the maturation of dendritic cells.