Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, A172 cells, Primary antibody: 162029(PDE4A Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Rabbit anti-Human PDE4A Polyclonal Antibody | anti-PDE4A antibody
PDE4A Rabbit pAb
IHC: 1:50-1:200
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, A172 cells, Primary antibody: 162029(PDE4A Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, A172 cells, Primary antibody: 162029(PDE4A Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162029(PDE4A Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162029(PDE4A Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 162029(PDE4A Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 162029(PDE4A Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))
NCBI and Uniprot Product Information
Uniprot Description
PDE4A: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2. Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum. Isoform 1, isoform 2, isoform 6 and isoform 7 are inhibited by rolipram and cilomilast. Isoform 1, isoform 2 and isoform 6 are inhibited by 4-[(3-butoxy-4-methoxyphenyl)-methyl]- 2-imidazolidinone (Ro 20-1724), roflumilast and denbufylline. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Phosphodiesterase; EC 3.1.4.53; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: membrane; perinuclear region of cytoplasm; cytosol
Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; protein binding; metal ion binding; cAMP binding
Biological Process: sensory perception of smell; cAMP catabolic process; signal transduction
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Product Notes
The PDE4A pde4a (Catalog #AAA4754858) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE4A Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDE4A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PDE4A pde4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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