Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) Standard Curve.)
Human Transient Receptor Potential Cation Channel Subfamily M Member 8 (TRPM8) ELISA Kit | TRPM8 elisa kit
Human Transient Receptor Potential Cation Channel Subfamily M Member 8 (TRPM8) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) Standard Curve.)
Background: TRPM8 is an ion channel, upon activation it allows the entry of Na+ (sodium) and Ca2+ (calcium) ions to the cell that leads to depolarization and the generation of an action potential. This eventually leads to the feeling of cold. The TRPM8 protein is expresed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin where as WS-12 and CPS-369 are the most selective agonist of TRPM8.TRPM8 is also expressed in the prostate, lungs, and bladder where its function is not very well understood.TRPM8 upregulation in bladder tissues correlates with pain in patients with painful bladder syndromes.Furthermore, TRPM8 is upregulated in many prostate cancer cell lines and Dendreon/Genentech are pursuing an agonist approach to induce apoptosis and prostate cancer cell death.
NCBI and Uniprot Product Information
Uniprot Description
TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: endoplasmic reticulum membrane; external side of plasma membrane; integral to membrane; lipid raft; plasma membrane
Molecular Function: calcium channel activity; protein binding; protein homodimerization activity
Biological Process: cellular calcium ion homeostasis; detection of temperature stimulus; protein homotetramerization; response to cold; thermoception
Research Articles on TRPM8
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Product Notes
The Human TRPM8 trpm8 (Catalog #AAA9713797) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713797 ELISA Kit recognizes Human TRPM8. It is sometimes possible for the material contained within the vial of "Transient Receptor Potential Cation Channel Subfamily M Member 8 (TRPM8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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