Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble Toll-like Receptor 9 (sTLR9/sCD289) Standard Curve.)
Mouse Soluble Toll-Like Receptor 9 (sTLR9/sCD289) ELISA Kit | sTLR9/sCD289 elisa kit
Mouse Soluble Toll-Like Receptor 9 (sTLR9/sCD289) ELISA Kit
Reactivity
Mouse
Synonyms
Soluble Toll-Like Receptor 9 (sTLR9/sCD289); Mouse Soluble Toll-Like Receptor 9 (sTLR9/sCD289) ELISA Kit; TLR9; CD289; sTLR9/sCD289 elisa kit
Reactivity
Mouse
Specificity
Mouse Soluble Toll-like Receptor 9 (sTLR9/sCD289) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TLR9. No significant cross-reactivity or interference between Mouse TLR9 and analogues was observed.
Sequence Length
1032
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble Toll-like Receptor 9 (sTLR9/sCD289) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Soluble Toll-like Receptor 9 (sTLR9/sCD289) Standard Curve.)
Related Product Information for sTLR9/sCD289 elisa kit
Description: This Mouse Soluble Toll-like Receptor 9 (sTLR9/sCD289) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR9 in samples. An antibody specific for TLR9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by TLR9 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR9 is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
Background: The protein encoded by TLR9 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR9 is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
toll-like receptor 9
NCBI Official Synonym Full Names
toll-like receptor 9
NCBI Official Symbol
Tlr9
NCBI Protein Information
toll-like receptor 9
UniProt Protein Name
Toll-like receptor 9
UniProt Gene Name
Tlr9
Uniprot Description
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:18931679, PubMed:21402738, PubMed:14993594, PubMed:17474149, PubMed:25686612, PubMed:18820679). Plays a role in defense against systemic mouse cytomegalovirus infection (PubMed:14993594). Controls lymphocyte response to Helicobacter infection (PubMed:17474149). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production ().
Research Articles on sTLR9/sCD289
Similar Products
Product Notes
The Mouse sTLR9/sCD289 tlr9 (Catalog #AAA9716816) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716816 ELISA Kit recognizes Mouse sTLR9/sCD289. It is sometimes possible for the material contained within the vial of "Soluble Toll-Like Receptor 9 (sTLR9/sCD289), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
