Mouse Toll-Like Receptor 3 (TLR3) ELISA Kit | TLR3 elisa kit

Mouse Toll-Like Receptor 3 (TLR3) ELISA Kit

Cat. Num. AAA9716820

• Gene Names: Tlr3; AI957183
• Reactivity: Mouse
• Synonyms: Toll-Like Receptor 3 (TLR3); Mouse Toll-Like Receptor 3 (TLR3) ELISA Kit; TLR3; CD283; TLR3 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Toll-like receptor 3 (TLR3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TLR3. No significant cross-reactivity or interference between Mouse TLR3 and analogues was observed.
• Sequence Length: 905

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Toll-like receptor 3 (TLR3) Standard Curve.)

Related Product Information for TLR3 elisa kit

Description: This Mouse Toll-like receptor 3 (TLR3) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR3 in samples. An antibody specific for TLR3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: TLR 3 is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. Discovered in 2001,TLR3 recognizes double-stranded RNA, a form of genetic information carried by some viruses such as reoviruses. Upon recognition, TLR 3 induces the activation of NF-kB to increase production of type I interferons which signal other cells to increase their antiviral defenses. Double-stranded RNA is also recognised by the cytoplasmic receptors RIG-I and MDA-5.The structure of TLR3 was reported in June 2005 by researchers at The Scripps Research Institute. TLR3 forms a large horseshoe shape that contacts with a neighboring horseshoe, forming a "dimer" of two horseshoes.

NCBI and Uniprot Product Information

• NCBI GI #: 31543872
• NCBI GeneID: 142980
• NCBI Accession #: NP_569054.2
• NCBI GenBank Nucleotide #: NP_569054.2
• UniProt Accession #: Q99MB1
• NCBI Official Full Name: toll-like receptor 3
• NCBI Official Synonym Full Names: toll-like receptor 3
• NCBI Official Symbol: Tlr3
• NCBI Official Synonym Symbols: AI957183
• NCBI Protein Information: toll-like receptor 3
• UniProt Protein Name: Toll-like receptor 3
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr3
• UniProt Entry Name: TLR3_MOUSE

Uniprot Description

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: cell surface; membrane; lysosomal membrane; endoplasmic reticulum; cytoplasm; integral to membrane; intracellular; endosome

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; RNA binding; double-stranded RNA binding; receptor activity

Biological Process: positive regulation of interleukin-12 production; positive regulation of JNK cascade; microglial cell activation; positive regulation of interferon-alpha biosynthetic process; defense response; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon type I production; positive regulation of interferon-beta production; inflammatory response; defense response to virus; positive regulation of cytokine production; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; MyD88-independent toll-like receptor signaling pathway; response to virus; response to dsRNA; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; positive regulation of chemokine biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; I-kappaB phosphorylation; positive regulation of inflammatory response; microglial cell activation during immune response; positive regulation of interferon-gamma biosynthetic process