Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Toll-like receptor 7 (TLR-7) Standard Curve.)
Human Toll-Like Receptor 7 (TLR-7) ELISA Kit | TLR-7 elisa kit
Human Toll-Like Receptor 7 (TLR-7) ELISA Kit
Gene Names
TLR7; TLR7-like
Reactivity
Human
Synonyms
Toll-Like Receptor 7 (TLR-7); Human Toll-Like Receptor 7 (TLR-7) ELISA Kit; TLR7; TLR-7 elisa kit
Reactivity
Human
Specificity
Human Toll-like receptor 7 (TLR-7) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLR7. No significant cross-reactivity or interference between Human TLR7 and analogues was observed.
Sequence Length
1049
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Toll-like receptor 7 (TLR-7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Toll-like receptor 7 (TLR-7) Standard Curve.)
Related Product Information for TLR-7 elisa kit
Description: This Human Toll-like receptor 7 (TLR-7) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR7 in samples. An antibody specific for TLR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Toll-like receptor 7is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. Imiquimod acts upon TLR 7. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.
Background: Toll-like receptor 7is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. Imiquimod acts upon TLR 7. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
toll-like receptor 7
NCBI Official Synonym Full Names
toll like receptor 7
NCBI Official Symbol
TLR7
NCBI Official Synonym Symbols
TLR7-like
NCBI Protein Information
toll-like receptor 7
UniProt Protein Name
Toll-like receptor 7
UniProt Gene Name
TLR7
UniProt Entry Name
TLR7_HUMAN
NCBI Description
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008]
Research Articles on TLR-7
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Product Notes
The Human TLR-7 tlr7 (Catalog #AAA9715982) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715982 ELISA Kit recognizes Human TLR-7. It is sometimes possible for the material contained within the vial of "Toll-Like Receptor 7 (TLR-7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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