Human Soluble Toll-Like Receptor 6 (TLR-6) ELISA Kit | TLR-6 elisa kit

Human Soluble Toll-Like Receptor 6 (TLR-6) ELISA Kit

Cat. Num. AAA9715983

• Gene Names: TLR6; CD286
• Reactivity: Human
• Synonyms: Soluble Toll-Like Receptor 6 (TLR-6); Human Soluble Toll-Like Receptor 6 (TLR-6) ELISA Kit; TLR6; CD286; OTTHUMP00000158730; TLR-6 elisa kit

• Reactivity: Human
• Specificity: Human Soluble Toll-like Receptor 6 (TLR-6) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLR6. No significant cross-reactivity or interference between Human TLR6 and analogues was observed.
• Sequence Length: 796

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Soluble Toll-like Receptor 6 (TLR-6) Standard Curve.)

Related Product Information for TLR-6 elisa kit

Description: This Human Soluble Toll-like Receptor 6 (TLR-6) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR6 in samples. An antibody specific for TLR6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Toll Like Receptor 6 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. By screening a human placenta library with a mouse Tlr6 probe, Takeuchi et al. (1999) isolated a cDNA encoding human TLR6. The deduced 796-amino acid type I transmembrane protein, which is 74% identical to mouse Tlr6 and 69% identical to TLR1, contains an N-terminal signal peptide, 20 tandemly repeated extracellular leucine-rich motifs, and a cytoplasmic domain homologous to IL1R, as seen in other TLRs. RT-PCR analysis detected Tlr6 in mouse thymus, spleen, ovary, and lung.

NCBI and Uniprot Product Information

• NCBI GI #: 20143971
• NCBI GeneID: 10333
• NCBI Accession #: NP_006059
• NCBI GenBank Nucleotide #: NP_006059.2
• UniProt Accession #: Q9Y2C9
• NCBI Official Full Name: toll-like receptor 6
• NCBI Official Synonym Full Names: toll like receptor 6
• NCBI Official Symbol: TLR6
• NCBI Official Synonym Symbols: CD286
• NCBI Protein Information: toll-like receptor 6
• UniProt Protein Name: Toll-like receptor 6
• UniProt Gene Name: TLR6
• UniProt Entry Name: TLR6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins. A Ser249Pro polymorphism in the extracellular domain of the encoded protein may be associated with an increased of asthma is some populations.[provided by RefSeq, Jan 2011]

Uniprot Description

TLR6: Participates in the innate immune response to Gram- positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. Belongs to the Toll-like receptor family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: phagocytic vesicle membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of JNK activity; T-helper 1 type immune response; regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; detection of diacylated bacterial lipoprotein; positive regulation of interleukin-6 biosynthetic process; activation of NF-kappaB-inducing kinase; signal transduction; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 6 signaling pathway; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; immune response; inflammatory response; toll-like receptor 4 signaling pathway