Rabbit anti-Human U2AF1 Polyclonal Antibody | anti-U2AF1 antibody

U2AF1, ID (Y114) (Splicing Factor U2AF 35kD Subunit, U2 Auxiliary Factor 35kD Subunit, U2 snRNP Auxiliary Factor Small Subunit, U2AF35, U2AFBP) (MaxLight 750)

Cat. Num. AAA6504329

• Gene Names: U2AF1; RN; FP793; U2AF35; U2AFBP; RNU2AF1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: U2AF1; Polyclonal Antibody; ID (Y114) (Splicing Factor U2AF 35kD Subunit; U2 Auxiliary Factor 35kD Subunit; U2 snRNP Auxiliary Factor Small Subunit; U2AF35; U2AFBP) (MaxLight 750); anti-U2AF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human U2AF1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-U2AF1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human U2AF1 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-U2AF1 Antibody (Center Y114) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with NRP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(U2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.)

Product Categories/Family for anti-U2AF1 antibody

Antibodies; Splicing Factors

References

1. Rino, J., Mol. Cell. Biol. 28(9), 3045-3057 (2008). 2. Little, J.T., J. Biol. Chem. 283(13), 8145-8152 (2008). 3. Pacheco, T.R., Mol. Cell. Biol. 26(21), 8183-8190 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5803207
• NCBI GeneID: 7307
• NCBI Accession #: NP_006749
• NCBI GenBank Nucleotide #: NM_006758
• UniProt Accession #: Q01081; Q701P4; Q71RF1
• Molecular Weight: 19,715 Da
• NCBI Official Full Name: splicing factor U2AF 35 kDa subunit isoform a
• NCBI Official Synonym Full Names: U2 small nuclear RNA auxiliary factor 1
• NCBI Official Symbol: U2AF1
• NCBI Official Synonym Symbols: RN; FP793; U2AF35; U2AFBP; RNU2AF1
• NCBI Protein Information: splicing factor U2AF 35 kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; splicin
• UniProt Protein Name: Splicing factor U2AF 35 kDa subunit
• Protein Family: Splicing factor
• UniProt Gene Name: U2AF1
• UniProt Synonym Gene Names: U2AF35; U2AFBP
• UniProt Entry Name: U2AF1_HUMAN

Uniprot Description

U2AF1: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. Belongs to the splicing factor SR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; spliceosome; Cajal body; nuclear speck

Molecular Function: RS domain binding; protein binding; metal ion binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription