Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Spondin-2 (SPON2) Standard Curve.)
Human Spondin-2 (SPON2) ELISA Kit | SPON2 elisa kit
Human Spondin-2 (SPON2) ELISA Kit
Gene Names
SPON2; DIL1; DIL-1; MINDIN; M-SPONDIN
Reactivity
Human
Synonyms
Spondin-2 (SPON2); Human Spondin-2 (SPON2) ELISA Kit; SPON2; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ34460; M-spondin; Mindin; SPON2 elisa kit
Reactivity
Human
Specificity
Human Spondin-2 (SPON2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPON2. No significant cross-reactivity or interference between Human SPON2 and analogues was observed.
Sequence Length
331
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Spondin-2 (SPON2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Spondin-2 (SPON2) Standard Curve.)
Related Product Information for SPON2 elisa kit
Description: This Human Spondin-2 (SPON2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPON2 in samples. An antibody specific for SPON2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPON2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPON2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPON2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The full-length SPON2 cDNA encodes a 331-amino acid protein with a domain organization similar to those of zebrafish mindin-1/mindin-2 and F-spondin (SPON1): a hydrophobic signal sequence in the N terminus, an FS1 domain, an FS2 domain, and a thrombospondin type I repeat. The mouse mindin protein, which is 85% identical to the human protein, is a conserved member of a family of secreted extracellular matrix proteins. RNA blot analysis detected expression in a variety of tissues, with abundant expression in lung and lymphoid tissues. Immunoblot blot analysis showed expression of a 42-kD protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.
Background: The full-length SPON2 cDNA encodes a 331-amino acid protein with a domain organization similar to those of zebrafish mindin-1/mindin-2 and F-spondin (SPON1): a hydrophobic signal sequence in the N terminus, an FS1 domain, an FS2 domain, and a thrombospondin type I repeat. The mouse mindin protein, which is 85% identical to the human protein, is a conserved member of a family of secreted extracellular matrix proteins. RNA blot analysis detected expression in a variety of tissues, with abundant expression in lung and lymphoid tissues. Immunoblot blot analysis showed expression of a 42-kD protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
spondin-2
NCBI Official Synonym Full Names
spondin 2
NCBI Official Symbol
SPON2
NCBI Official Synonym Symbols
DIL1; DIL-1; MINDIN; M-SPONDIN
NCBI Protein Information
spondin-2
UniProt Protein Name
Spondin-2
Protein Family
UniProt Gene Name
SPON2
UniProt Synonym Gene Names
DIL1; DIL-1
UniProt Entry Name
SPON2_HUMAN
Uniprot Description
SPON2: Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern- recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 4p16.3
Cellular Component: extracellular space; proteinaceous extracellular matrix
Molecular Function: lipopolysaccharide binding; metal ion binding; antigen binding
Biological Process: induction of bacterial agglutination; mast cell mediated immunity; axon guidance; innate immune response; positive regulation of interleukin-6 production; opsonization; cell adhesion; positive regulation of tumor necrosis factor production; defense response to fungus; defense response to virus
Research Articles on SPON2
Similar Products
Product Notes
The Human SPON2 spon2 (Catalog #AAA9714005) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714005 ELISA Kit recognizes Human SPON2. It is sometimes possible for the material contained within the vial of "Spondin-2 (SPON2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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