Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Microtubule-associated protein RP/EB family member 3 (MAPRE3) Standard Curve.)
Mouse Microtubule-Associated Protein RP/EB Family Member 3 (MAPRE3) ELISA Kit | MAPRE3 elisa kit
Mouse Microtubule-Associated Protein RP/EB Family Member 3 (MAPRE3) ELISA Kit
Gene Names
Mapre3; EB2; EB3; RP3; EBF3; AI790651
Reactivity
Mouse
Synonyms
Microtubule-Associated Protein RP/EB Family Member 3 (MAPRE3); Mouse Microtubule-Associated Protein RP/EB Family Member 3 (MAPRE3) ELISA Kit; MAPRE3; EB3; EBF3; EBF3-S; RP3; APC binding protein; MAPRE3 elisa kit
Reactivity
Mouse
Specificity
Mouse Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MAPRE3. No significant cross-reactivity or interference between Mouse MAPRE3 and analogues was observed.
Sequence Length
281
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Microtubule-associated protein RP/EB family member 3 (MAPRE3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Microtubule-associated protein RP/EB family member 3 (MAPRE3) Standard Curve.)
Related Product Information for MAPRE3 elisa kit
Description: This Mouse Microtubule-associated protein RP/EB family member 3 (MAPRE3) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPRE3 in samples. An antibody specific for MAPRE3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAPRE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPRE3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPRE3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAPRE3 is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. The predicted 282-amino acid protein is 54% identical to MAPRE1. Northern blot analysis revealed expression of a 2.2-kb transcript predominantly in brain and muscle. GST pull-down analysis determined that a homologous region in the C termini of APC and APCL binds to MAPRE3. Immunofluorescence and confocal microscopy demonstrated that MAPRE3 is localized in the microtubule network and colocalizes with APCL in the perinucleus and microtubule network.
Background: MAPRE3 is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. The predicted 282-amino acid protein is 54% identical to MAPRE1. Northern blot analysis revealed expression of a 2.2-kb transcript predominantly in brain and muscle. GST pull-down analysis determined that a homologous region in the C termini of APC and APCL binds to MAPRE3. Immunofluorescence and confocal microscopy demonstrated that MAPRE3 is localized in the microtubule network and colocalizes with APCL in the perinucleus and microtubule network.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated protein RP/EB family member 3 isoform 1
NCBI Official Synonym Full Names
microtubule-associated protein, RP/EB family, member 3
NCBI Official Symbol
Mapre3
NCBI Official Synonym Symbols
EB2; EB3; RP3; EBF3; AI790651
NCBI Protein Information
microtubule-associated protein RP/EB family member 3
UniProt Protein Name
Microtubule-associated protein RP/EB family member 3
Protein Family
UniProt Gene Name
Mapre3
UniProt Synonym Gene Names
EBF3; EB3
UniProt Entry Name
MARE3_MOUSE
Research Articles on MAPRE3
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Product Notes
The Mouse MAPRE3 mapre3 (Catalog #AAA9717732) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717732 ELISA Kit recognizes Mouse MAPRE3. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Protein RP/EB Family Member 3 (MAPRE3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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