Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated protein RP/EB family member 1 (MAPRE1) Standard Curve.)
Human Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1) ELISA Kit | MAPRE1 elisa kit
Human Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated protein RP/EB family member 1 (MAPRE1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated protein RP/EB family member 1 (MAPRE1) Standard Curve.)
Background: MAPRE1 is a regulator of microtubule dynamics that localizes at both the growing plus ends of microtubules and the centrosome. It is involved in a variety of cellular processes, including establishment and maintenance of cell polarity, search and capture of chromosomes during mitosis, and positioning of the mitotic spindle during asymmetric cell division. This microtubule localization was abolished in the presence of the microtubule-destabilizing drug nocodazole; upon drug removal, the microtubule distribution of EB1 was recovered and EB1 fluorescence concentrated at the microtubule-organizing center. These results suggested that EB1 is associated with the microtubule network and may be involved in microtubule polymerization and spindle function.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]
Uniprot Description
EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 20q11.1-q11.23
Cellular Component: Golgi apparatus; centrosome; microtubule; cell projection membrane; cytoplasmic microtubule; cytoplasm; cortical microtubule cytoskeleton; spindle; cytosol
Molecular Function: protein C-terminus binding; microtubule plus-end binding; protein binding
Biological Process: mitosis; cell proliferation; cell division; organelle organization and biogenesis; negative regulation of microtubule polymerization; mitotic cell cycle; G2/M transition of mitotic cell cycle
Research Articles on MAPRE1
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Product Notes
The Human MAPRE1 mapre1 (Catalog #AAA9715389) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715389 ELISA Kit recognizes Human MAPRE1. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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