Rabbit DDX6 Polyclonal Antibody | anti-DDX6 antibody

Anti-DDX6 Antibody

Cat. Num. AAA416651

• Gene Names: DDX6; P54; RCK; HLR2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: DDX6; Polyclonal Antibody; Anti-DDX6 Antibody; anti-DDX6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 6246

• Applicable Applications for anti-DDX6 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: 1:40,000-80,000; Western blot analysis: 1:200-500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-DDX6 antibody

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

References

(1) Nonhoff,U., Ralser,M., Welzel,F., et al. Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and Interferes with P-bodies and stress granules. Mol. Biol. Cell 2007;18 (4):1385-1396

NCBI and Uniprot Product Information

• NCBI GI #: 164664517
• NCBI GeneID: 1656
• NCBI Accession #: NP_004388.2
• NCBI GenBank Nucleotide #: NM_004397
• Molecular Weight: 54,417 Da
• NCBI Official Full Name: Homo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 6 (DDX6), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 6
• NCBI Official Symbol: DDX6
• NCBI Official Synonym Symbols: P54; RCK; HLR2
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX6; ATP-dependent RNA helicase p54; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); oncogene RCK
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX6
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX6
• UniProt Entry Name: DDX6_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]

Uniprot Description

DDX6: an oncogenic ATP-dependent helicase of the DEAD box family. Interacts with argonaute proteins, Ago1 and Ago2, and is a general repressor of translation in human cells by miRNA-induced gene silencing. In the process of mRNA degradation, may play a role in mRNA decapping.

Protein type: RNA processing; EC 3.6.4.13; RNA-binding; Oncoprotein; Helicase

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; helicase activity; ATP binding; RNA helicase activity

Biological Process: gene expression; cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay