Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated protein RP/EB family member 2 (MAPRE2) Standard Curve.)
Human Microtubule-Associated Protein RP/EB Family Member 2 (MAPRE2) ELISA Kit | MAPRE2 elisa kit
Human Microtubule-Associated Protein RP/EB Family Member 2 (MAPRE2) ELISA Kit
Gene Names
MAPRE2; EB1; EB2; RP1; CSCSC2
Reactivity
Human
Synonyms
Microtubule-Associated Protein RP/EB Family Member 2 (MAPRE2); Human Microtubule-Associated Protein RP/EB Family Member 2 (MAPRE2) ELISA Kit; MAPRE2; EB1; EB2; RP1; APC-binding protein EB1; T-cell activation protein; EB1 family; MAPRE2 elisa kit
Reactivity
Human
Specificity
Human Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPRE2. No significant cross-reactivity or interference between Human MAPRE2 and analogues was observed.
Sequence Length
327
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated protein RP/EB family member 2 (MAPRE2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated protein RP/EB family member 2 (MAPRE2) Standard Curve.)
Related Product Information for MAPRE2 elisa kit
Description: This Human Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPRE2 in samples. An antibody specific for MAPRE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAPRE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPRE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPRE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAPRE2 shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. The deduced 327-amino acid protein has significant homology with EB1 family proteins. Northern blot analysis detected a 2.6-kb transcript in T cells activated by 2 signals (i.e., cell surface antigen(s) and/or cytokine) and also in lymphocyte tumor cell lines. Immunoprecipitation analysis indicated that RP1 associates with full-length but not C terminus-deleted APC.
Background: MAPRE2 shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. The deduced 327-amino acid protein has significant homology with EB1 family proteins. Northern blot analysis detected a 2.6-kb transcript in T cells activated by 2 signals (i.e., cell surface antigen(s) and/or cytokine) and also in lymphocyte tumor cell lines. Immunoprecipitation analysis indicated that RP1 associates with full-length but not C terminus-deleted APC.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated protein RP/EB family member 2 isoform 1
NCBI Official Synonym Full Names
microtubule associated protein RP/EB family member 2
NCBI Official Symbol
MAPRE2
NCBI Official Synonym Symbols
EB1; EB2; RP1; CSCSC2
NCBI Protein Information
microtubule-associated protein RP/EB family member 2
UniProt Protein Name
Microtubule-associated protein RP/EB family member 2
Protein Family
UniProt Gene Name
MAPRE2
UniProt Synonym Gene Names
RP1; EB2
UniProt Entry Name
MARE2_HUMAN
NCBI Description
The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Uniprot Description
RP1: shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. Its function is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 18q12.1
Cellular Component: microtubule cytoskeleton; cytoplasm
Molecular Function: microtubule binding
Biological Process: cell proliferation; mitosis; cell division; signal transduction
Research Articles on MAPRE2
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Product Notes
The Human MAPRE2 mapre2 (Catalog #AAA9715388) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715388 ELISA Kit recognizes Human MAPRE2. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Protein RP/EB Family Member 2 (MAPRE2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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