Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Microtubule-associated protein RP/EB family member 1 (MAPRE1) Standard Curve.)
Rat Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1) ELISA Kit | MAPRE1 elisa kit
Rat Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1) ELISA Kit
Gene Names
Mapre1; Eb1
Reactivity
Rat
Synonyms
Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1); Rat Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1) ELISA Kit; MAPRE1; EB1; MGC117374; MGC129946; adenomatous polyposis coli-binding protein EB1; MAPRE1 elisa kit
Reactivity
Rat
Specificity
Rat Microtubule-associated protein RP/EB family member 1 (MAPRE1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAPRE1. No significant cross-reactivity or interference between Rat MAPRE1 and analogues was observed.
Sequence Length
268
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Microtubule-associated protein RP/EB family member 1 (MAPRE1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Microtubule-associated protein RP/EB family member 1 (MAPRE1) Standard Curve.)
Related Product Information for MAPRE1 elisa kit
Description: This Rat Microtubule-associated protein RP/EB family member 1 (MAPRE1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPRE1 in samples. An antibody specific for MAPRE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAPRE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPRE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPRE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAPRE1 is a regulator of microtubule dynamics that localizes at both the growing plus ends of microtubules and the centrosome. It is involved in a variety of cellular processes, including establishment and maintenance of cell polarity, search and capture of chromosomes during mitosis, and positioning of the mitotic spindle during asymmetric cell division. This microtubule localization was abolished in the presence of the microtubule-destabilizing drug nocodazole; upon drug removal, the microtubule distribution of EB1 was recovered and EB1 fluorescence concentrated at the microtubule-organizing center. These results suggested that EB1 is associated with the microtubule network and may be involved in microtubule polymerization and spindle function.
Background: MAPRE1 is a regulator of microtubule dynamics that localizes at both the growing plus ends of microtubules and the centrosome. It is involved in a variety of cellular processes, including establishment and maintenance of cell polarity, search and capture of chromosomes during mitosis, and positioning of the mitotic spindle during asymmetric cell division. This microtubule localization was abolished in the presence of the microtubule-destabilizing drug nocodazole; upon drug removal, the microtubule distribution of EB1 was recovered and EB1 fluorescence concentrated at the microtubule-organizing center. These results suggested that EB1 is associated with the microtubule network and may be involved in microtubule polymerization and spindle function.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated protein RP/EB family member 1
NCBI Official Synonym Full Names
microtubule-associated protein, RP/EB family, member 1
NCBI Official Symbol
Mapre1
NCBI Official Synonym Symbols
Eb1
NCBI Protein Information
microtubule-associated protein RP/EB family member 1
UniProt Protein Name
Microtubule-associated protein RP/EB family member 1
Protein Family
UniProt Gene Name
Mapre1
UniProt Synonym Gene Names
EB1
UniProt Entry Name
MARE1_RAT
NCBI Description
expressed specifically at growing microtubule distal tips [RGD, Feb 2006]
Uniprot Description
EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.
Protein type: Microtubule-binding; Cell cycle regulation; Cytoskeletal; Motility/polarity/chemotaxis
Cellular Component: microtubule cytoskeleton; Golgi apparatus; microtubule; centrosome; cell projection; cytoplasmic microtubule; cell projection membrane; cytoplasm; microtubule organizing center; cortical microtubule cytoskeleton; spindle
Molecular Function: microtubule plus-end binding
Biological Process: mitosis; cell division; negative regulation of microtubule polymerization; regulation of microtubule polymerization
Research Articles on MAPRE1
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Product Notes
The Rat MAPRE1 mapre1 (Catalog #AAA9712326) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712326 ELISA Kit recognizes Rat MAPRE1. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Protein RP/EB Family Member 1 (MAPRE1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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