Mouse IGFBP5 ELISA Kit | IGFBP5 elisa kit

Mouse IGFBP5 PicoKine ELISA Kit

Cat. Num. AAA177283

• Gene Names: Igfbp5; IGFBP-5; AI256729; AW208790; IGFBP-5P
• Reactivity: Mouse
• Synonyms: IGFBP5; Mouse IGFBP5 PicoKine ELISA Kit; Insulin-like growth factor-binding protein 5; insulin-like growth factor binding protein 5; IBP 5; IBP-5; IBP5; IBP5_HUMAN; IGF binding protein 5; IGF BP5; IGF-binding protein 5; IGFBP 5; IGFBP-5; Insulin like growth factor binding protein 5; IGFBP5 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse IGFBP5
• Sequence Length: 271

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse IGFBP5 PicoKine ELISA Kit standard curve )

Related Product Information for IGFBP5 elisa kit

Principle of the assay: mouse IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP5 has been precoated onto 96-well plates. Standards(NSO, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP5 amount of sample captured in plate.
Background: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 585294
• NCBI GeneID: 16011
• UniProt Accession #: Q07079
• Molecular Weight: 30,372 Da
• NCBI Official Full Name: Insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: Igfbp5
• NCBI Official Synonym Symbols: IGFBP-5; AI256729; AW208790; IGFBP-5P
• NCBI Protein Information: insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp5
• UniProt Synonym Gene Names: Igfbp-5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Entry Name: IBP5_MOUSE

Uniprot Description

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted; Cell cycle regulation; Secreted, signal peptide

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; growth factor binding; fibronectin binding; insulin-like growth factor II binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; mammary gland involution; hair follicle morphogenesis; negative regulation of smooth muscle cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; glucose metabolic process; negative regulation of smooth muscle cell migration; glucose homeostasis; positive regulation of protein kinase B signaling cascade; negative regulation of translation; regulation of growth; striated muscle cell differentiation; negative regulation of osteoblast differentiation; regulation of cell growth; negative regulation of cell migration