Human SPINK1/TATI ELISA Kit | SPINK1 elisa kit

Human SPINK1/TATI PicoKine ELISA Kit

Cat. Num. AAA177146

• Gene Names: SPINK1; TCP; PCTT; PSTI; TATI; Spink3
• Reactivity: Human
• Synonyms: SPINK1/TATI; Human SPINK1/TATI PicoKine ELISA Kit; Pancreatic secretory trypsin inhibitor; serine peptidase inhibitor; Kazal type 1; ISK1_HUMAN; Pancreatic serine protease inhibitor; Kazal; PCTT; PSTI; Serine protease inhibitor Kazal type 1; Serine protease inhibitor Kazal-type 1; Spink 3; SPINK1; Spink3; TaTI; TCP; Tumor associated trypsin inhibitor; Tumor-associated trypsin inhibitor; SPINK1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human SPINK1
• Sequence Length: 79

• Samples: Cell culture supernates, serum and plasma (heparin)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human SPINK1/TATI PicoKine ELISA Kit standard curve )

Related Product Information for SPINK1 elisa kit

Principle of the assay: human SPINK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPINK1 has been precoated onto 96-well plates. Standards(E.coli, D24-C79) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPINK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SPINK1 amount of sample captured in plate.
Background: Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.

NCBI and Uniprot Product Information

• NCBI GI #: 124856
• NCBI GeneID: 6690
• UniProt Accession #: P00995
• Molecular Weight: 8,507 Da
• NCBI Official Full Name: Pancreatic secretory trypsin inhibitor
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kazal type 1
• NCBI Official Symbol: SPINK1
• NCBI Official Synonym Symbols: TCP; PCTT; PSTI; TATI; Spink3
• NCBI Protein Information: pancreatic secretory trypsin inhibitor; tumor-associated trypsin inhibitor; serine protease inhibitor, Kazal type 1
• UniProt Protein Name: Pancreatic secretory trypsin inhibitor
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: SPINK1
• UniProt Synonym Gene Names: PSTI; TATI
• UniProt Entry Name: ISK1_HUMAN

NCBI Description

The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008]

Uniprot Description

SPINK1: This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP). TCP is an idiopathic, juvenile, nonalcoholic form of chronic pancreatitis widely prevalent in several tropical countries. It can be associated with fibrocalculous pancreatic diabetes (FCPD) depending on both environmental and genetic factors. TCP differs from alcoholic pancreatitis by a much younger age of onset, pancreatic calcification, a high incidence of insulin dependent but ketosis resistant diabetes mellitus, and an exceptionally high incidence of pancreatic cancer.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; regulation of acrosome reaction

Disease: Tropical Calcific Pancreatitis; Pancreatitis, Hereditary