Typical Testing Data/Standard Curve (for reference only)
(Mouse Cathepsin D PicoKine ELISA Kit standard curve )
Mouse Cathepsin D ELISA Kit | CTSD elisa kit
Mouse Cathepsin D PicoKine ELISA Kit
Gene Names
Ctsd; CD; CatD
Reactivity
Mouse
Synonyms
Cathepsin D; Mouse Cathepsin D PicoKine ELISA Kit; cathepsin D; CATD_HUMAN; Cathepsin D heavy chain; Ceroid lipofuscinosis neuronal 10; CLN10; CPSD; ctsd; Lysosomal aspartyl protease; CTSD elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse Cathepsin D
Sequence Length
410
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse Cathepsin D PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse Cathepsin D PicoKine ELISA Kit standard curve )
Related Product Information for CTSD elisa kit
Principle of the assay: mouse Cathepsin D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cathepsin D has been precoated onto 96-well plates. Standards(NSO, I21-L410) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cathepsin D amount of sample captured in plate.
Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
44,954 Da
NCBI Official Full Name
Cathepsin D
NCBI Official Synonym Full Names
cathepsin D
NCBI Official Symbol
Ctsd
NCBI Official Synonym Symbols
CD; CatD
NCBI Protein Information
cathepsin D
UniProt Protein Name
Cathepsin D
Protein Family
UniProt Gene Name
Ctsd
UniProt Entry Name
CATD_MOUSE
Uniprot Description
CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family.
Protein type: Protease; Autophagy; EC 3.4.23.5; Motility/polarity/chemotaxis
Cellular Component: extracellular matrix; extracellular space; mitochondrion; lysosome; extracellular region
Molecular Function: peptidase activity; hydrolase activity; endopeptidase activity; aspartic-type endopeptidase activity; peptide binding
Biological Process: proteolysis; autophagic vacuole formation
Research Articles on CTSD
Similar Products
Product Notes
The Mouse CTSD ctsd (Catalog #AAA177314) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177314 ELISA Kit recognizes Mouse CTSD. It is sometimes possible for the material contained within the vial of "Cathepsin D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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