Typical Testing Data/Standard Curve (for reference only)
(Human REG-4 PicoKine ELISA Kit standard curve )
Human REG-4 ELISA Kit | REG4 elisa kit
Human REG-4 PicoKine ELISA Kit
Gene Names
REG4; GISP; RELP; REG-IV
Reactivity
Human
Synonyms
REG-4; Human REG-4 PicoKine ELISA Kit; Regenerating islet-derived protein 4; regenerating islet-derived family; member 4; Gastrointestinal secretory protein; GISP; REG IV; REG like protein; REG-like protein; Reg4; REG4_HUMAN; Regenerating gene type IV; Regenerating islet derived family member 4; Regenerating islet derived protein 4 precursor; Regenerating islet-derived protein IV; REGIV; RELP; REG4 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human REG-4
Sequence Length
158
Samples
Cell culture supernates, serum and plasma (heparin)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human REG-4 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human REG-4 PicoKine ELISA Kit standard curve )
Related Product Information for REG4 elisa kit
Principle of the assay: human REG-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for REG-4 has been precoated onto 96-well plates. Standards(E.coli, D23-P158) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for REG-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human REG-4 amount of sample captured in plate.
Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.
Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,993 Da
NCBI Official Full Name
Regenerating islet-derived protein 4
NCBI Official Synonym Full Names
regenerating islet-derived family, member 4
NCBI Official Symbol
REG4
NCBI Official Synonym Symbols
GISP; RELP; REG-IV
NCBI Protein Information
regenerating islet-derived protein 4; REG-4; reg IV; REG-like protein; regenerating gene type IV; gastrointestinal secretory protein; regenerating islet-derived protein IV
UniProt Protein Name
Regenerating islet-derived protein 4
Protein Family
UniProt Gene Name
REG4
UniProt Synonym Gene Names
GISP; RELP; REG-4; Reg IV
UniProt Entry Name
REG4_HUMAN
Uniprot Description
REG4: Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p13.1-p12
Cellular Component: cytoplasm; extracellular region
Molecular Function: heparin binding; calcium ion binding
Research Articles on REG4
Similar Products
Product Notes
The Human REG4 reg4 (Catalog #AAA177355) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177355 ELISA Kit recognizes Human REG4. It is sometimes possible for the material contained within the vial of "REG-4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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