Typical Testing Data/Standard Curve (for reference only)
Mouse Fatty Acid Transport Protein 1 (FATP1) ELISA Kit | FATP1 elisa kit
Mouse Fatty Acid Transport Protein 1 (FATP1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FATP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FATP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FATP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FATP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
SLC27A1: Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. The LFCA import appears to be hormone-regulated in a tissue-specific manner. In adipocytes, but not myocytes, insulin induces a rapid translocation of FATP1 from intracellular compartments to the plasma membrane, paralleled by increased LFCA uptake. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane- associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LFCA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. Belongs to the ATP-dependent AMP-binding enzyme family.
Protein type: EC 6.2.1.-; Ligase; Lipid-binding; Membrane protein, integral; Mitochondrial; Vesicle
Chromosomal Location of Human Ortholog: 8|8 B3.3
Cellular Component: caveola; cytoplasm; cytoplasmic vesicle; endoplasmic reticulum; integral component of membrane; intracellular membrane-bound organelle; membrane; mitochondrial inner membrane; mitochondrion; plasma membrane
Molecular Function: catalytic activity; fatty acid transmembrane transporter activity; ligase activity; long-chain-fatty-acid-CoA ligase activity; nucleotide binding; protein homodimerization activity; very long-chain fatty acid-CoA ligase activity
Biological Process: adiponectin-activated signaling pathway; cardiolipin biosynthetic process; fatty acid metabolic process; fatty acid transport; lipid metabolic process; lipid transport; long-chain fatty acid metabolic process; long-chain fatty acid transport; medium-chain fatty acid transport; metabolic process; phosphatidic acid biosynthetic process; phosphatidylcholine biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylglycerol biosynthetic process; phosphatidylinositol biosynthetic process; phosphatidylserine biosynthetic process; positive regulation of heat generation; response to cold; response to insulin stimulus; transport
Research Articles on FATP1
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Product Notes
The Mouse FATP1 slc27a1 (Catalog #AAA456857) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456857 ELISA Kit recognizes Mouse FATP1. It is sometimes possible for the material contained within the vial of "Fatty Acid Transport Protein 1 (FATP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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