Human Mucin-7 ELISA Kit | MUC7 elisa kit

Human Mucin-7 ELISA Kit

Cat. Num. AAA2886134

• Gene Names: MUC7; MG2
• Reactivity: Human
• Synonyms: Mucin-7; Human Mucin-7 ELISA Kit; MUC-7; Apo-MG2; Salivary mucin-7; MUC7; MG2; MUC7 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Mucin-7,MUC-7
• Sequence Length: 377

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.469-30 ng/mL
• Sensitivity: <0.12 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.1%
• Inter-assay Precision: Inter-Assay CV: <=6.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUC7 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 222418647
• NCBI GeneID: 4589
• NCBI Accession #: NP_001138478.1
• NCBI GenBank Nucleotide #: NM_001145006.1
• UniProt Accession #: Q8TAX7; Q9UCD7; Q9UCD8
• Molecular Weight: 39,159 Da
• NCBI Official Full Name: mucin-7
• NCBI Official Synonym Full Names: mucin 7, secreted
• NCBI Official Symbol: MUC7
• NCBI Official Synonym Symbols: MG2
• NCBI Protein Information: mucin-7
• UniProt Protein Name: Mucin-7
• Protein Family: Mucin
• UniProt Gene Name: MUC7
• UniProt Synonym Gene Names: MG2; MUC-7
• UniProt Entry Name: MUC7_HUMAN

NCBI Description

This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. This antimicrobial protein has antibacterial and antifungal activity. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

MUC7: May function in a protective capacity by promoting the clearance of bacteria in the oral cavity and aiding in mastication, speech, and swallowing. Binds P.aeruginosa pili. Genetic variations in MUC7 are associated with susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: Golgi lumen

Molecular Function: protein binding

Biological Process: cellular protein metabolic process; O-glycan processing; post-translational protein modification; protein amino acid O-linked glycosylation

Disease: Asthma, Susceptibility To