Rat Long-chain fatty acid transport protein 1 ELISA Kit | Slc27a1 elisa kit

Rat Long-chain fatty acid transport protein 1 ELISA Kit

Cat. Num. AAA764761

• Gene Names: SLC27A1; FATP; FATP1; ACSVL5; FATP-1
• Reactivity: Rat
• Synonyms: Long-chain fatty acid transport protein 1; Rat Long-chain fatty acid transport protein 1 ELISA Kit; Slc27a1/SLC27A1/ACSVL5FATP-1/EC 6.2.1/EC 6.2.1.-/FATP1Solute carrier family 27 member 1/FATPlong-chain fatty acid transport protein 1/Fatty acid transport protein 1/FLJ00336/MGC71751/solute carrier family 27 (fatty acid transporter); member 1; Slc27a1 elisa kit

• Reactivity: Rat
• Sequence Length: 646

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Slc27a1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Slc27a1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Slc27a1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Slc27a1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Slc27a1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38524616
• NCBI GeneID: 376497
• NCBI Accession #: NP_940982.1
• NCBI GenBank Nucleotide #: NM_198580.2
• Molecular Weight: 51,780 Da
• NCBI Official Full Name: long-chain fatty acid transport protein 1
• NCBI Official Synonym Full Names: solute carrier family 27 member 1
• NCBI Official Symbol: SLC27A1
• NCBI Official Synonym Symbols: FATP; FATP1; ACSVL5; FATP-1
• NCBI Protein Information: long-chain fatty acid transport protein 1
• UniProt Protein Name: Long-chain fatty acid transport protein 1
• Protein Family: Long-chain fatty acid transport protein
• UniProt Gene Name: SLC27A1
• UniProt Synonym Gene Names: ACSVL5; FATP1; FATP-1; Fatty acid transport protein 1
• UniProt Entry Name: S27A1_HUMAN

Uniprot Description

SLC27A1: Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. The LFCA import appears to be hormone-regulated in a tissue-specific manner. In adipocytes, but not myocytes, insulin induces a rapid translocation of FATP1 from intracellular compartments to the plasma membrane, paralleled by increased LFCA uptake. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane- associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LFCA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. Belongs to the ATP-dependent AMP-binding enzyme family.

Protein type: Vesicle; Lipid-binding; Membrane protein, integral; Mitochondrial; EC 6.2.1.-; Ligase

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: caveola; endoplasmic reticulum; integral to membrane; membrane; mitochondrial inner membrane; plasma membrane

Molecular Function: fatty acid transporter activity; long-chain-fatty-acid-CoA ligase activity; nucleotide binding; protein homodimerization activity; very-long-chain-fatty-acid-CoA ligase activity

Biological Process: adiponectin-mediated signaling pathway; cardiolipin biosynthetic process; cellular lipid metabolic process; long-chain fatty acid metabolic process; long-chain fatty acid transport; medium-chain fatty acid transport; phosphatidic acid biosynthetic process; phosphatidylcholine biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylglycerol biosynthetic process; phosphatidylinositol biosynthetic process; phosphatidylserine biosynthetic process; positive regulation of heat generation; response to cold; response to insulin stimulus; transmembrane transport