Rabbit anti-Human, Mouse NFYB Polyclonal Antibody | anti-NFYB antibody

NFYB Antibody

Cat. Num. AAA9216352

• Gene Names: NFYB; HAP3; CBF-A; CBF-B; NF-YB
• Reactivity: Human, Mouse
• Applications: Western Blot
• Synonyms: NFYB; Polyclonal Antibody; NFYB Antibody; Purified Rabbit Polyclonal Antibody (Pab); Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB; HAP3; anti-NFYB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Form/Format: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Sequence Length: 207

• Applicable Applications for anti-NFYB antibody: Western Blot (WB)
• Application Notes: WB ~~1:1000
• Antigen Source: Human
• Antigen Type: A Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-NFYB Antibody at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NFYB antibody

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'- CCAAT-3' box motif found in the promoters of its target genes. NF- Y can function as both an activator and a repressor, depending on its interacting cofactors.

Product Categories/Family for anti-NFYB antibody

Signal Transduction

References

Li X.-Y.,et al.J. Biol. Chem. 267:8984-8990(1992). Badley Clarke J.,et al.Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases. Li X.-Y.,et al.Nucleic Acids Res. 20:1087-1091(1992). Ota T.,et al.Nat. Genet. 36:40-45(2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 5453780
• NCBI GeneID: 4801
• NCBI Accession #: NP_006157.1
• NCBI GenBank Nucleotide #: NM_006166.3
• UniProt Accession #: P25208; Q96IY8; A8K7B9
• Molecular Weight: 22831
• NCBI Official Full Name: nuclear transcription factor Y subunit beta
• NCBI Official Synonym Full Names: nuclear transcription factor Y subunit beta
• NCBI Official Symbol: NFYB
• NCBI Official Synonym Symbols: HAP3; CBF-A; CBF-B; NF-YB
• NCBI Protein Information: nuclear transcription factor Y subunit beta
• UniProt Protein Name: Nuclear transcription factor Y subunit beta
• Protein Family: Nuclear transcription factor
• UniProt Gene Name: NFYB
• UniProt Synonym Gene Names: HAP3; NF-YB
• UniProt Entry Name: NFYB_HUMAN

NCBI Description

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]

Uniprot Description

NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; protein complex binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent