Human Fatty Acid Transport Protein 4 (FATP4) RTU ELISA Kit | FATP4 rtu elisa kit

Human Fatty Acid Transport Protein 4 (FATP4) ELISA Kit

Cat. Num. AAA459212

• Gene Names: SLC27A4; IPS; FATP4; ACSVL4
• Reactivity: Human
• Synonyms: Fatty Acid Transport Protein 4 (FATP4); Human Fatty Acid Transport Protein 4 (FATP4) ELISA Kit; SLC27A4; FATP4; Long-Chain Fatty Acid Transport Protein 4; Solute Carrier Family 27 Member 4; Fatty Acid Transporter; FATP4 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FABP2. No significant cross-reactivity or interference between FABP2 and analogues was observed.
• Sequence Length: 643

• Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.063ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FABP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FABP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FATP4 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FABP2 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FABP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FABP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 40807357
• NCBI GeneID: 10999
• NCBI Accession #: NP_005085.2
• NCBI GenBank Nucleotide #: NM_005094.3
• UniProt Accession #: Q6P1M0; O95186; Q96G53; A8K2F7
• Molecular Weight: 26,001 Da
• NCBI Official Full Name: long-chain fatty acid transport protein 4
• NCBI Official Synonym Full Names: solute carrier family 27 member 4
• NCBI Official Symbol: SLC27A4
• NCBI Official Synonym Symbols: IPS; FATP4; ACSVL4
• NCBI Protein Information: long-chain fatty acid transport protein 4
• UniProt Protein Name: Long-chain fatty acid transport protein 4
• UniProt Gene Name: SLC27A4
• UniProt Synonym Gene Names: ACSVL4; FATP4; FATP-4; Fatty acid transport protein 4

NCBI Description

This gene encodes a member of a family of fatty acid transport proteins, which are involved in translocation of long-chain fatty acids cross the plasma membrane. This protein is expressed at high levels on the apical side of mature enterocytes in the small intestine, and appears to be the principal fatty acid transporter in enterocytes. Clinical studies suggest this gene as a candidate gene for the insulin resistance syndrome. Mutations in this gene have been associated with ichthyosis prematurity syndrome. [provided by RefSeq, Apr 2010]

Uniprot Description

Involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. Appears to be the principal fatty acid transporter in small intestinal enterocytes. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids (VLCFAs). Indirectly inhibits RPE65 via substrate competition and via production of VLCFA derivatives like lignoceroyl-CoA. Prevents light-induced degeneration of rods and cones ().