Porcine Fatty Acid Binding Protein 1, Liver (FABP1) ELISA Kit | FABP1 elisa kit

Pig Fatty Acid Binding Protein 1, Liver (FABP1) ELISA Kit

Cat. Num. AAA2703445

• Gene Names: FABP1; FABPL; L-FABP
• Reactivity: Porcine
• Synonyms: Fatty Acid Binding Protein 1; Liver (FABP1); Pig Fatty Acid Binding Protein 1; Liver (FABP1) ELISA Kit; FABP-1; FABPL; L-FABP; LFABP; Liver-type fatty acid-binding protein; FABP1 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 1, Liver (FABP1).; No significant cross-reactivity or interference between Fatty Acid Binding Protein 1, Liver (FABP1) and analogues was observed.
• Sequence Length: 127

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.134ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 1, Liver (FABP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 1, Liver (FABP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FABP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 1, Liver (FABP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 1, Liver (FABP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 1, Liver (FABP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Binding Protein 1, Liver (FABP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21618454
• NCBI GeneID: 2168
• UniProt Accession #: P07148
• Molecular Weight: 14,208 Da
• NCBI Official Full Name: Fatty acid binding protein 1, liver
• NCBI Official Synonym Full Names: fatty acid binding protein 1
• NCBI Official Symbol: FABP1
• NCBI Official Synonym Symbols: FABPL; L-FABP
• NCBI Protein Information: fatty acid-binding protein, liver
• UniProt Protein Name: Fatty acid-binding protein, liver
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP1
• UniProt Synonym Gene Names: FABPL; L-FABP

NCBI Description

This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011]

Uniprot Description

Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PubMed:25732850). Binds cholesterol (PubMed:25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport ().