Human DAN/NBL1 PicoKine ELISA Kit | NBL1 elisa kit

Human DAN/NBL1 PicoKine ELISA Kit

Cat. Num. AAA177625

• Gene Names: NBL1; NB; DAN; NO3; DAND1; D1S1733E
• Reactivity: Human
• Synonyms: DAN/NBL1 PicoKine; Human DAN/NBL1 PicoKine ELISA Kit; Neuroblastoma suppressor of tumorigenicity 1; D1S1733E; DAN; DAN domain family member 1; DAND 1; DAND1; Differential screening selected gene aberrant in neuroblastoma; MGC8972; NB; NBL 1; Neuroblastoma candidate region suppression of tumorigenicity 1; Neuroblastoma suppression of tumorigenicity 1; NO 3; NO3; Zinc finger protein DAN ; neuroblastoma 1; DAN family BMP antagonist; NBL1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human DAN
• Sequence Length: 181

• Assay Range: 125pg/ml-8000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human DAN in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, A17-D181
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human DAN standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human DAN standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|1257|3204|5394|1020|3562|4975; Standard Deviation|72.90|189.03|334.42|65.28|235.09|353.22; CV(%)|5.8|5.9|6.2|6.4|6.6|7.1
• Sample Data: Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000; O.D.|0.019|0.095|0.191|0.389|0.697|1.274|1.845|2.124
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human DAN/NBL1 PicoKine ELISA Kit standard curve )

Related Product Information for NBL1 elisa kit

Principle of the assay: Human DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D181) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DAN amount of sample captured in plate.

Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

NCBI and Uniprot Product Information

• NCBI GI #: 323276675
• NCBI GeneID: 4681
• NCBI Accession #: NP_001191013.1
• NCBI GenBank Nucleotide #: NM_001204084.2
• UniProt Accession #: P41271; Q5TGZ2; Q5U0N4; Q96L68; A3KFI7
• Molecular Weight: 23,169 Da
• NCBI Official Full Name: neuroblastoma suppressor of tumorigenicity 1 isoform 2
• NCBI Official Synonym Full Names: neuroblastoma 1, DAN family BMP antagonist
• NCBI Official Symbol: NBL1
• NCBI Official Synonym Symbols: NB; DAN; NO3; DAND1; D1S1733E
• NCBI Protein Information: neuroblastoma suppressor of tumorigenicity 1
• UniProt Protein Name: Neuroblastoma suppressor of tumorigenicity 1
• Protein Family: N-terminal-borealin-like protein
• UniProt Gene Name: NBL1
• UniProt Synonym Gene Names: DAN; DAND1
• UniProt Entry Name: NBL1_HUMAN

NCBI Description

This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]

Uniprot Description

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.