NBL1 active protein

NBL1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123070

• Gene Names: Nbl1; DAN; NO3; Dana; D4H1S1733E
• Purity: >95% as determined by SDS-PAGE
• Synonyms: NBL1; NBL1 Protein; Mouse; Recombinant (His Tag); Mouse NBL1/DAND1/DAN Protein (His Tag); D4H1S1733E Protein; DAN Protein; Dana Protein; NO3 Protein; neuroblastoma 1; DAN family BMP antagonist; NBL1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp178

• Species: Mouse
• Activity: Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells. The ED50 for this effect is 3-9ug/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse NBL1 (Q61477)(Met 1-Asp 178) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.The ED50 for this effect is 3-9 ug/mL.)

Related Product Information for NBL1 active protein

Background: The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in the vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression, and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante, and PRDC (protein related to DAN and Cerberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in the regulation of the entry of cells into the S phase. Besides, the DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumor suppressor gene for neuroblastoma.

References

Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.

NCBI and Uniprot Product Information

• NCBI GI #: 148234601
• NCBI GeneID: 17965
• NCBI Accession #: NP_032701.2
• NCBI GenBank Nucleotide #: NM_008675.2
• UniProt Accession #: Q61477; Q499K5
• Molecular Weight: 19,107 Da
• NCBI Official Full Name: neuroblastoma suppressor of tumorigenicity 1
• NCBI Official Synonym Full Names: neuroblastoma, suppression of tumorigenicity 1
• NCBI Official Symbol: Nbl1
• NCBI Official Synonym Symbols: DAN; NO3; Dana; D4H1S1733E
• NCBI Protein Information: neuroblastoma suppressor of tumorigenicity 1
• UniProt Protein Name: Neuroblastoma suppressor of tumorigenicity 1
• Protein Family: N-terminal-borealin-like protein
• UniProt Gene Name: Nbl1
• UniProt Synonym Gene Names: Dan; Dana
• UniProt Entry Name: NBL1_MOUSE

Uniprot Description

NBL1: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Secreted; Secreted, signal peptide

Cellular Component: extracellular region

Molecular Function: morphogen activity; protein binding; protein homodimerization activity

Biological Process: determination of dorsal identity; negative regulation of BMP signaling pathway; nervous system development; neurite morphogenesis; positive regulation of neuron differentiation