Rabbit anti-Rat CNGA3 Polyclonal Antibody | anti-CNGA3 antibody

CNGA3 (Cyclic Nucleotide-gated Cation Channel alpha 3, CNGa2, CCNC1)

Cat. Num. AAA612262

• Gene Names: CNGA3; CNG3; ACHM2; CCNC1; CCNCa; CNCG3; CCNCalpha
• Reactivity: Rat
• Applications: Western Blot
• Purity: Purified; Purified
• Synonyms: CNGA3; Polyclonal Antibody; CNGA3 (Cyclic Nucleotide-gated Cation Channel alpha 3; CNGa2; CCNC1); Anti -CNGA3 (Cyclic Nucleotide-gated Cation Channel alpha 3; anti-CNGA3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Homology with other species
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-CNGA3 antibody: Western Blot (WB)
• Application Notes: Western blotting of rat brain membranes: 1. Anti-CNGA3 antibody (1:200), 2. Anti-CNGA3 antibody, preincubated with the control peptide antigen., Control antigen included
• Immunogen: Peptide (C)GHGFSPDRENSEDASKAD, corresponding to amino acid residues 594-611 of rat CNGA3 (accession number Q9QWN7).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blotting of rat brain membrane lysate: 1. MBS612262, 1:200. 2. MBS612262, preincubated with the control peptide antigen.)

Related Product Information for anti-CNGA3 antibody

Cyclic nucleotides are important second messengers in many cellular functions such as visual transduction, and relaxation of smooth muscle cells. Cyclic nucleotides exert their cellular functions through three major classes of cellular receptors, one of them is the cyclic nucleotide-gated (CNG) channels. 1 The CNG channels are non-selective cation channels facilitating the influx of Na +and Ca 2+ ions, following activation by intracellular cyclic nucleotides. 2In vertebrates, six members of the CNG channel family were identified and grouped according to sequence homology into two subtypes, CNGA and CNGB. The native CNG channels are composed of alpha and beta subunits in a tetrameric configuration. To date, four types of the alpha subunits (CNGA1-4) and two beta subunits (CNGB1, CNGB3) have been characterized. 3-4 Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains but, are also modulated by other factors including phosphorylation and calmodulin. 5In a heterologous expression system, only the alpha subunits are capable of forming functional homomeric channels. CNG ion channels are essential in visual and olfactory signal transduction. The CNGA3 channel is primarily expressed in cone photoreceptors where it participates in light transduction (daylight and color vision). CNG channels expression was also found in the hippocampus, cortex, Purkinje cells and other neural derived tissues. CNGA3 was also detected in kidney, heart, brain and sperm, where it was thought to have an important role in the control of sperm motility and fertility. However, CNGA3-deficient mice were shown to be as fertile and produce at normal rate as wild types. 6

Product Categories/Family for anti-CNGA3 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 120433600
• NCBI GeneID: 1261
• NCBI Accession #: NP_001073347.1
• NCBI GenBank Nucleotide #: NM_001079878.1
• UniProt Accession #: Q16281; Q4VAP7; Q53RD2; Q9UP64
• Molecular Weight: 78,838 Da
• NCBI Official Full Name: cyclic nucleotide-gated cation channel alpha-3 isoform 2
• NCBI Official Synonym Full Names: cyclic nucleotide gated channel alpha 3
• NCBI Official Symbol: CNGA3
• NCBI Official Synonym Symbols: CNG3; ACHM2; CCNC1; CCNCa; CNCG3; CCNCalpha
• NCBI Protein Information: cyclic nucleotide-gated cation channel alpha-3; CNG-3; CNG channel alpha-3; cyclic nucleotide-gated channel alpha-3; cone photoreceptor cGMP-gated channel alpha subunit; cone photoreceptor cGMP-gated channel subunit alpha
• UniProt Protein Name: Cyclic nucleotide-gated cation channel alpha-3
• Protein Family: Cyclic nucleotide-gated cation channel
• UniProt Gene Name: CNGA3
• UniProt Synonym Gene Names: CNCG3; CNG channel alpha-3; CNG-3; CNG3
• UniProt Entry Name: CNGA3_HUMAN

NCBI Description

This gene encodes a member of the cyclic nucleotide-gated cation channel protein family which is required for normal vision and olfactory signal transduction. Mutations in this gene are associated with achromatopsia (rod monochromacy) and color blindness. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

CNGA3: Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones. Defects in CNGA3 are the cause of achromatopsia type 2 (ACHM2); also known as total colorblindness or rod monochromacy (RMCH2). ACHM2 is an autosomal recessive condition characterized by day blindness and photophobia. In ACHM2 patients the cones are defective and the subjects see better at night. Defects in CNGA3 may be a cause of Leber congenital amaurosis (LCA), a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near- absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: integral to plasma membrane; cytoplasm; dendrite; perikaryon

Molecular Function: protein C-terminus binding; voltage-gated potassium channel activity; intracellular cAMP activated cation channel activity; intracellular cGMP activated cation channel activity; cGMP binding; ligand-gated ion channel activity

Biological Process: phototransduction, visible light; regulation of membrane potential; response to magnesium ion; response to cAMP; retinal cone cell development; visual perception; transport; signal transduction; cation transport; response to corticosteroid stimulus

Disease: Achromatopsia 2