Human NBL1 ELISA Kit | NBL1 elisa kit

Human NBL1 ELISA Kit

Cat. Num. AAA8248379

• Gene Names: NBL1; NB; DAN; NO3; DAND1; D1S1733E
• Reactivity: Human
• Synonyms: NBL1; Human NBL1 ELISA Kit; DAN; DAND1; Neuroblastoma suppressor of tumorigenicity 1; DAN domain family member 1; Protein N03; Zinc finger protein DAN; NBL1 elisa kit

• Reactivity: Human
• Specificity: The Human NBL1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human NBL1 proteins within the range of 125 pg/ml - 8000 pg/ml.
• Sequence Length: 181

• Samples: Cell culture supernates, serum, plasma
• Sensitivity: < 10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NBL1 elisa kit

Principle of the Assay: The MyBioSource' Human NBL1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human NBL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NBL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NBL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

NCBI and Uniprot Product Information

• NCBI GI #: 323276675
• NCBI GeneID: 4681
• NCBI Accession #: NP_001191013.1
• NCBI GenBank Nucleotide #: NM_001204084.2
• UniProt Accession #: P41271; Q5TGZ2; Q5U0N4; Q96L68; A3KFI7
• Molecular Weight: 23,169 Da
• NCBI Official Full Name: neuroblastoma suppressor of tumorigenicity 1 isoform 2
• NCBI Official Synonym Full Names: neuroblastoma 1, DAN family BMP antagonist
• NCBI Official Symbol: NBL1
• NCBI Official Synonym Symbols: NB; DAN; NO3; DAND1; D1S1733E
• NCBI Protein Information: neuroblastoma suppressor of tumorigenicity 1
• UniProt Protein Name: Neuroblastoma suppressor of tumorigenicity 1
• Protein Family: N-terminal-borealin-like protein
• UniProt Gene Name: NBL1
• UniProt Synonym Gene Names: DAN; DAND1
• UniProt Entry Name: NBL1_HUMAN

NCBI Description

This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]

Uniprot Description

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.