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Western Blot (WB) (CALU Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CALU antibody detected the CALU protein (arrow).)

Rabbit anti-Human CALU Polyclonal Antibody | anti-CALU antibody

CALU, CT (CALU, Calumenin, Crocalbin, IEF SSP 9302) (APC)

Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CALU; Polyclonal Antibody; CT (CALU; Calumenin; Crocalbin; IEF SSP 9302) (APC); IEF SSP 9302; anti-CALU antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-CALU antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CALU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human CALU.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CALU Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CALU antibody detected the CALU protein (arrow).)

Western Blot (WB) (CALU Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CALU antibody detected the CALU protein (arrow).)

Immunohistochemistry (IHC)

(CALU Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CALU Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (CALU Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CALU Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-CALU antibody
The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified.
Product Categories/Family for anti-CALU antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Carlquist, J.F., et al. J. Thromb. Thrombolysis 30(3):358-364(2010). Hernandez-Romero, D., et al. Ann. Med. 42(6):439-446(2010). Harada, T., et al. Thromb. Res. 126(3):183-190(2010). Voora, D., et al. Clin. Pharmacol. Ther. 87(4):445-451(2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
813
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,455 Da
NCBI Official Full Name
calumenin isoform b
NCBI Official Synonym Full Names
calumenin
NCBI Official Symbol
CALU
NCBI Protein Information
calumenin; IEF SSP 9302; crocalbin-like protein; multiple EF-hand protein
UniProt Protein Name
Calumenin
Protein Family
UniProt Gene Name
CALU
UniProt Entry Name
CALU_HUMAN

NCBI Description

The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]

Uniprot Description

CALU: Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity. Belongs to the CREC family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q32.1

Cellular Component: Golgi apparatus; sarcoplasmic reticulum lumen; membrane; endoplasmic reticulum; extracellular region; melanosome

Molecular Function: protein binding; calcium ion binding

Biological Process: platelet activation; platelet degranulation; blood coagulation

Research Articles on CALU

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Product Notes

The CALU calu (Catalog #AAA6280574) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CALU, CT (CALU, Calumenin, Crocalbin, IEF SSP 9302) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CALU can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CALU calu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CALU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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