Mouse CXCL17 ELISA Kit | CXCL17 elisa kit

Mouse CXCL17 PicoKine ELISA Kit

Cat. Num. AAA1752564

• Gene Names: Cxcl17; Vcc1; VCC-1
• Reactivity: Mouse
• Synonyms: CXCL17; Mouse CXCL17 PicoKine ELISA Kit; Mouse CXCL17; chemokine (C-X-C motif) ligand 17; C-X-C motif chemokine 17; 6-Cys CXCL17; 13.6 kDa protein; VEGF coregulated chemokine 1; 4-Cys CXCL17; Cxcl17; Vcc1; CXCL17 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Cxcl17
• Sequence Length: 119

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CXCL17 elisa kit

Principle of the Assay: The Picokine™ Mouse Cxcl17 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Cxcl17 with a 96-well strip plate that is pre-coated with antibody specific for Cxcl17. The detection antibody is a biotinylated antibody specific for Cxcl17. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Cxcl17 with immunogen: Expression system for standard: E.coli, Immunogen sequence: S23-L119. The kit is analytically validated with ready to use reagents. To measure Mouse Cxcl17, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Cxcl17 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Cxcl17 in the sample.!!Background/Introduction: Chemokine (C-X-C motif) ligand 17 (CXCL17) is a small cytokine belonging to the CXC chemokine family that has been identified in humans and mice. It is mapped to 7; 7 A3. The protein encoded by this gene is a mucosal chemokine that attracts immature dendritic cells and blood monocytes to the lungs. The encoded protein also promotes tumorigenesis through an angiogenic activity. Finally, this protein exhibits strong antimicrobial activity against E. coli, S. aureus, Salmonella, P. aeruginosa, and C. albicans. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 133891727
• NCBI GeneID: 232983
• NCBI Accession #: NP_705804.2
• NCBI GenBank Nucleotide #: NP_705804.2
• UniProt Accession #: Q5UW37
• NCBI Official Full Name: C-X-C motif chemokine 17
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 17
• NCBI Official Symbol: Cxcl17
• NCBI Official Synonym Symbols: Vcc1; VCC-1
• NCBI Protein Information: C-X-C motif chemokine 17
• UniProt Protein Name: C-X-C motif chemokine 17
• Protein Family: VEGF coregulated chemokine
• UniProt Gene Name: Cxcl17
• UniProt Synonym Gene Names: Vcc1; 6-Cys CXCL17

Uniprot Description

Chemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells (PubMed:24973458). Plays a role in angiogenesis and possibly in the development of tumors (PubMed:16989774). Acts as an anti-inflammatory in the stomach. May play a role in the innate defense against infections. Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions.