Mouse CCL9 ELISA Kit | Ccl9 elisa kit

Mouse CCL9 PicoKine ELISA Kit

Cat. Num. AAA177190

• Gene Names: Ccl9; CCF18; MRP-2; Scya9; Scya10
• Reactivity: Mouse
• Synonyms: CCL9; Mouse CCL9 PicoKine ELISA Kit; C-C motif chemokine 9; chemokine (C-C motif) ligand 9; CCF18; Chemokine CC motif ligand 9; Macrophage Inflammatory Protein 1 gamma; Macrophage inflammatory protein-related protein 2; MIP-1 gamma; mMIP1g; SCYA10; SCYA9; Small inducible cytokine A9; Ccl9 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse CCL8
• Sequence Length: 122

• Samples: Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, Edta)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ccl9 elisa kit

Principle of the Assay: The Picokine Mouse CCL8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse CCL8 with a 96-well strip plate that is pre-coated with antibody specific for CCL8. The detection antibody is a biotinylated antibody specific for CCL8. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse CCL8 with immunogen: Expression system for standard: E.coli; Immunogen sequence: E20-P97. The kit is analytically validated with ready to use reagents. To measure Mouse CCL8, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse CCL8 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse CCL8 in the sample!!Background/Introduction: Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 1709026
• NCBI GeneID: 20308
• UniProt Accession #: P51670; Q5QNW2
• Molecular Weight: 13,871 Da
• NCBI Official Full Name: C-C motif chemokine 9
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 9
• NCBI Official Symbol: Ccl9
• NCBI Official Synonym Symbols: CCF18; MRP-2; Scya9; Scya10
• NCBI Protein Information: C-C motif chemokine 9; MIP-1 gamma; small inducible cytokine A9; small inducible cytokine A10; macrophage inflammatory protein 1-gamma; macrophage inflammatory protein-related protein 2
• UniProt Protein Name: C-C motif chemokine 9
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl9
• UniProt Synonym Gene Names: Mrp2; Scya10; Scya9; MIP-1-gamma; MRP-2
• UniProt Entry Name: CCL9_MOUSE

Uniprot Description

CCL9: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; cytokine activity

Biological Process: immune response; negative regulation of myoblast differentiation; chemotaxis