Rabbit anti-Human KISS1 Polyclonal Antibody | anti-KISS1 antibody

KISS1 (Kisspeptin 1, Malignant Melanoma Metastasis Suppressor, HH13, KiSS-1) (FITC)

Cat. Num. AAA6423947

• Gene Names: KISS1; HH13; KiSS-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: KISS1; Polyclonal Antibody; KISS1 (Kisspeptin 1; Malignant Melanoma Metastasis Suppressor; HH13; KiSS-1) (FITC); anti-KISS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KISS1.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-KISS1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:20-1:50; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human KISS1.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of Mouse brain tissue lysate, using KISS1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human brain tissue using KISS1 antibody.)

Product Categories/Family for anti-KISS1 antibody

Antibodies; Tumor Suppressors

NCBI and Uniprot Product Information

• NCBI GI #: 292495092
• NCBI GeneID: 3814
• UniProt Accession #: Q15726; Q9HBP1; A8K6N0
• Molecular Weight: 138
• NCBI Official Full Name: Metastasis-suppressor KiSS-1
• NCBI Official Synonym Full Names: KiSS-1 metastasis-suppressor
• NCBI Official Symbol: KISS1
• NCBI Official Synonym Symbols: HH13; KiSS-1
• NCBI Protein Information: metastasis-suppressor KiSS-1; metastin; kisspeptin-1; prepro-kisspeptin; malignant melanoma metastasis-suppressor
• UniProt Protein Name: Metastasis-suppressor KiSS-1
• Protein Family: Metastasis-suppressor
• UniProt Gene Name: KISS1
• UniProt Entry Name: KISS1_HUMAN

NCBI Description

This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Mar 2012]

Uniprot Description

KiSS-1: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell- matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine- tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Belongs to the KISS1 family.

Protein type: Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular region

Molecular Function: protein binding

Biological Process: cytoskeleton organization and biogenesis

Disease: Hypogonadotropic Hypogonadism 13 With Or Without Anosmia