Mouse CD23 ELISA Kit | FCER2 elisa kit

Mouse CD23 ELISA Kit

Cat. Num. AAA8248435

• Gene Names: Fcer2a; CD23; Fce2; Fcer2; Ly-42
• Reactivity: Mouse
• Synonyms: CD23; Mouse CD23 ELISA Kit; CD23A; CLEC4J; FCE2; IGEBF; Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; FCER2 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse CD23 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD23 proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 330

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <15 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FCER2 elisa kit

Principle of the Assay: The Mouse CD23 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD23 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse CD23 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: CD23, also known as Fc epsilon RII, or FcepsilonRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

NCBI and Uniprot Product Information

• NCBI GI #: 359339166
• NCBI GeneID: 14128
• NCBI Accession #: NP_001240666.1
• NCBI GenBank Nucleotide #: NM_001253737.1
• UniProt Accession #: P20693; Q61556; Q61557
• Molecular Weight: 37,487 Da
• NCBI Official Full Name: low affinity immunoglobulin epsilon Fc receptor isoform B
• NCBI Official Synonym Full Names: Fc receptor, IgE, low affinity II, alpha polypeptide
• NCBI Official Symbol: Fcer2a
• NCBI Official Synonym Symbols: CD23; Fce2; Fcer2; Ly-42
• NCBI Protein Information: low affinity immunoglobulin epsilon Fc receptor
• UniProt Protein Name: Low affinity immunoglobulin epsilon Fc receptor
• Protein Family: Low affinity immunoglobulin epsilon Fc receptor
• UniProt Gene Name: Fcer2
• UniProt Synonym Gene Names: Fcer2a
• UniProt Entry Name: FCER2_MOUSE

Uniprot Description

FCER2: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

Protein type: Membrane protein, integral

Cellular Component: external side of plasma membrane

Biological Process: positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of killing of cells of another organism; positive regulation of nitric-oxide synthase activity; positive regulation of nitric-oxide synthase biosynthetic process