Typical Testing Data/Standard Curve (for reference only)
(Human CD23/FCER2 PicoKine ELISA Kit standard curve )
Human CD23/FCER2 ELISA Kit | FCER2 elisa kit
Human CD23/FCER2 PicoKine ELISA Kit
Gene Names
FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
Reactivity
Human
Synonyms
CD23/FCER2; Human CD23/FCER2 PicoKine ELISA Kit; Low affinity immunoglobulin epsilon Fc receptor; Fc fragment of IgE; low affinity II; receptor for (CD23); BLAST 2; BLAST-2; C-type lectin domain family 4 member J; CD23; CD23 antigen; CD23A; CLEC4J; Fc fragment of IgE low affinity II receptor for (CD23); Fc-epsilon-RII; FCE2; Fcer2; FCER2_HUMAN; IGEBF; Immunoglobulin E-binding factor; Low affinity immunoglobulin epsilon Fc receptor soluble form; Lymphocyte IgE receptor; FCER2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CD23
Sequence Length
320
Samples
Cell culture supernates, serum, plasma (heparin, EDTA) and urine
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human CD23/FCER2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human CD23/FCER2 PicoKine ELISA Kit standard curve )
Related Product Information for FCER2 elisa kit
Principle of the assay: human CD23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD23 has been precoated onto 96-well plates. Standards(NSO, D48-S321) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD23 amount of sample captured in plate.
Background: CD23, also known as Fc epsilon RII, or FcepsilonRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23(FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
Background: CD23, also known as Fc epsilon RII, or FcepsilonRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23(FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
36,299 Da
NCBI Official Synonym Full Names
Fc fragment of IgE, low affinity II, receptor for (CD23)
NCBI Official Symbol
FCER2
NCBI Official Synonym Symbols
CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
NCBI Protein Information
low affinity immunoglobulin epsilon Fc receptor; CD23 antigen; fc-epsilon-RII; lymphocyte IgE receptor; immunoglobulin E-binding factor; C-type lectin domain family 4, member J
UniProt Protein Name
Low affinity immunoglobulin epsilon Fc receptor membrane-bound form
Protein Family
UniProt Gene Name
FCER2
UniProt Entry Name
K3W4U1_HUMAN
NCBI Description
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Research Articles on FCER2
Similar Products
Product Notes
The Human FCER2 fcer2 (Catalog #AAA176555) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA176555 ELISA Kit recognizes Human FCER2. It is sometimes possible for the material contained within the vial of "CD23/FCER2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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