Typical Testing Data/Standard Curve (for reference only)
Mouse C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit | ROS1 elisa kit
Mouse C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ROS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ROS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ROS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ROS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ROS: a proto-oncogenic receptor tyrosine kinase whose expression is tightly restricted during development. Normally expressed in adult murine and human epithelial cells of the epididymis. Transgenic mice lacking the c-ros gene are infertile. Ectopic expression of c-Ros has been reported in meningiomas and astrocytomas. Is up-regulated in human glioma: 30% of malignant glioma tumors are ROS positive. An oncogenic fusion protein between PIST (aka FIG) and ROS, resulting from an intra-chromosomal homozygous deletion of 240 kilobases on 6q21, is found in glioblastoma multiform. PIST (aka FIG) is a peripheral membrane protein associated with the Golgi apparatus. Unlike other fusion RTK oncogenes, the mechanism of activation of PIST-ROS does not appear to be dimerization: the PIST-ROS fusion protein appears to be monomeric in vivo. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region of PIST, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. c-ROS may also be activated epigenetically, suggesting caution when using 5-aza-dC for treating glioma.
Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); Sev family; TK group
Chromosomal Location of Human Ortholog: 10 B3|10 26.18 cM
Cellular Component: cell surface; integral component of membrane; membrane; perinuclear region of cytoplasm; plasma membrane
Molecular Function: ATP binding; kinase activity; nucleotide binding; protein kinase activity; protein phosphatase binding; protein tyrosine kinase activity; transferase activity; transmembrane receptor protein tyrosine kinase activity
Biological Process: cell differentiation; cell proliferation; columnar/cuboidal epithelial cell development; negative regulation of gene expression; peptidyl-tyrosine autophosphorylation; phosphorylation; protein amino acid phosphorylation; regulation of cell growth; regulation of ERK1 and ERK2 cascade; regulation of phosphate transport; regulation of TOR signaling; spermatogenesis
Research Articles on ROS1
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Product Notes
The Mouse ROS1 ros1 (Catalog #AAA456919) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456919 ELISA Kit recognizes Mouse ROS1. It is sometimes possible for the material contained within the vial of "C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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