Typical Testing Data/Standard Curve (for reference only)
Bovine c-ros oncogene 1, receptor tyrosine kinase (ROS1) ELISA Kit | ROS1 elisa kit
Bovine c-ros oncogene 1, receptor tyrosine kinase (ROS1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ROS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ROS1 antibody and an ROS1-HRP conjugate. The assay sample and buffer are incubated together with ROS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ROS1 concentration since ROS1 from samples and ROS1-HRP conjugate compete for the anti-ROS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ROS1 from the sample, fewer sites are left to bind ROS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ROS1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
ROS: a proto-oncogenic receptor tyrosine kinase whose expression is tightly restricted during development. Normally expressed in adult murine and human epithelial cells of the epididymis. Transgenic mice lacking the c-ros gene are infertile. Ectopic expression of c-Ros has been reported in meningiomas and astrocytomas. Is up-regulated in human glioma: 30% of malignant glioma tumors are ROS positive. An oncogenic fusion protein between PIST (aka FIG) and ROS, resulting from an intra-chromosomal homozygous deletion of 240 kilobases on 6q21, is found in glioblastoma multiform. PIST (aka FIG) is a peripheral membrane protein associated with the Golgi apparatus. Unlike other fusion RTK oncogenes, the mechanism of activation of PIST-ROS does not appear to be dimerization: the PIST-ROS fusion protein appears to be monomeric in vivo. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region of PIST, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. c-ROS may also be activated epigenetically, suggesting caution when using 5-aza-dC for treating glioma.
Protein type: EC 2.7.10.1; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; Oncoprotein; TK group; Sev family
Chromosomal Location of Human Ortholog: 6q22
Cellular Component: membrane; plasma membrane; integral to membrane
Molecular Function: protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding
Biological Process: cell proliferation; peptidyl-tyrosine phosphorylation; spermatogenesis; cell differentiation; columnar/cuboidal epithelial cell development; cell growth; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of TOR signaling pathway
Research Articles on ROS1
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Product Notes
The Bovine ROS1 ros1 (Catalog #AAA7232591) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7232591 ELISA Kit recognizes Bovine ROS1. It is sometimes possible for the material contained within the vial of "c-ros oncogene 1, receptor tyrosine kinase (ROS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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