Mouse BMP7 ELISA Kit | BMP7 elisa kit

Mouse BMP7 ELISA Kit

Cat. Num. AAA8248421

• Gene Names: Bmp7; OP1
• Reactivity: Mouse
• Synonyms: BMP7; Mouse BMP7 ELISA Kit; OP1; Bone morphogenetic protein 7; BMP-7; Osteogenic protein 1; OP-1; Eptotermin alfa; BMP7 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse BMP7 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse BMP7 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 430

• Samples: Bone Tissue, Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP7 elisa kit

Principle of the Assay: The Mouse BMP7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse BMP7 in Bone Tissue, Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse BMP7 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse BMP7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

NCBI and Uniprot Product Information

• NCBI GI #: 31982487
• NCBI GeneID: 12162
• NCBI Accession #: NP_031583.2
• NCBI GenBank Nucleotide #: NM_007557.3
• UniProt Accession #: P23359; Q91XF7
• Molecular Weight: 49,199 Da
• NCBI Official Full Name: bone morphogenetic protein 7 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: Bmp7
• NCBI Official Synonym Symbols: OP1
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp7
• UniProt Synonym Gene Names: Bmp-7; Op1; BMP-7; OP-1
• UniProt Entry Name: BMP7_MOUSE

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. Mutation of this gene results in skeletal, kidney, and other developmental defects. [provided by RefSeq, Jul 2016]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; vesicle

Molecular Function: cytokine activity; heparin binding; protein binding; transforming growth factor beta receptor binding

Biological Process: anatomical structure formation; axon guidance; BMP signaling pathway; branching morphogenesis of a tube; camera-type eye morphogenesis; cardiac muscle development; cell development; embryonic camera-type eye morphogenesis; embryonic limb morphogenesis; embryonic pattern specification; epithelial cell differentiation; eye development; hindbrain development; kidney development; mesenchymal cell differentiation; mesoderm formation; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of MAP kinase activity; negative regulation of mitosis; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of Notch signaling pathway; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; neural fold elevation formation; neurite morphogenesis; odontogenesis of dentine-containing teeth; organ morphogenesis; pattern specification process; pharyngeal system development; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell differentiation; positive regulation of heterotypic cell-cell adhesion; positive regulation of neuron differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of MAPKKK cascade; regulation of phosphorylation; salivary gland morphogenesis; steroid hormone mediated signaling; tube morphogenesis; ureteric bud development