Mouse ANGPTL3 ELISA Kit | ANGPTL3 elisa kit

Mouse ANGPTL3 PicoKine ELISA Kit

Cat. Num. AAA177160

• Gene Names: Angptl3; hypl
• Reactivity: Mouse
• Synonyms: ANGPTL3; Mouse ANGPTL3 PicoKine ELISA Kit; Angiopoietin-related protein 3; angiopoietin-like 3; ANG 5; ANG-5; ANG5; Angiopoietin 5; Angiopoietin like 3; Angiopoietin related protein 3; Angiopoietin-5; Angiopoietin-like protein 3; ANGL3_HUMAN; ANGPT5; ANL3; FHBL2; OTTHUMP00000010719; ANGPTL3 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse ANGPTL3
• Sequence Length: 455

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse ANGPTL3 PicoKine ELISA Kit standard curve )

Related Product Information for ANGPTL3 elisa kit

Principle of the assay: mouse ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-T455) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ANGPTL3 amount of sample captured in plate.
Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

NCBI and Uniprot Product Information

• NCBI GI #: 25008124
• NCBI GeneID: 30924
• UniProt Accession #: Q9R182
• Molecular Weight: 52,543 Da
• NCBI Official Full Name: Angiopoietin-related protein 3
• NCBI Official Synonym Full Names: angiopoietin-like 3
• NCBI Official Symbol: Angptl3
• NCBI Official Synonym Symbols: hypl
• NCBI Protein Information: angiopoietin-related protein 3; hypolipidemia; angiopoietin-like protein 3
• UniProt Protein Name: Angiopoietin-related protein 3
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: Angptl3
• UniProt Entry Name: ANGL3_MOUSE

NCBI Description

This gene encodes a member of the angiopoietin-like family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may inhibit triglyceride metabolism. Homozygous knockout mice for this gene exhibit reduced plasma lipid concentrations, including reduced plasma triglyceride concentrations, and enhanced activity of enzymes involved in triglyceride metabolism. [provided by RefSeq, Aug 2015]

Uniprot Description

ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Inhibitor

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome; extracellular region

Molecular Function: integrin binding; enzyme inhibitor activity; phospholipase inhibitor activity; growth factor activity

Biological Process: cholesterol metabolic process; phospholipid catabolic process; glycerol metabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; fatty acid metabolic process; signal transduction; positive regulation of angiogenesis; sequestering of lipid; cholesterol homeostasis; triacylglycerol metabolic process; acylglycerol homeostasis; phospholipid metabolic process; negative regulation of catalytic activity; artery morphogenesis; positive regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; positive regulation of cell migration; phospholipid homeostasis