Toll Like Receptor 7 (TLR7) Recombinant Protein | TLR7 recombinant protein

Recombinant Toll Like Receptor 7 (TLR7)

Cat. Num. AAA2018425

• Gene Names: TLR7; TLR7-like
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Toll Like Receptor 7 (TLR7); Recombinant Toll Like Receptor 7 (TLR7); TLR7 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose
• Concentration: Original Concentration: 100ug/mL (varies by lot)
• Sequence: The sequence of the target protein is listed below. ; PDCC YDAFIVYDTK DPAVTEWVLA ELVAKLEDPR EKHFNLCLEE RDWLPGQPVL ENLSQSIQLS KKTVFVMTDK YAKTENFKIA FYLSHQRLMD EKVDVIILIF LEKPFQKSKF LQLRKRLCGS SVLEWPTNPQ AHPYFWQCLK NALATD
• Sequence Length: 1049

• Applicable Applications for TLR7 recombinant protein: Positive Conrtrol, Immunogen, SDS-PAGE, Western Blot (WB); ; (May be suitable for use in other assays to be deetermined by the end user.)
• Expression System: Prokaryotic expression
• Residues: Pro887~Asp1036 (Accession # Q9NYK1) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Membrane, Plasma
• Traits: Freeze-dried powder
• Predicted Isoelectric Point: 6.8
• Reconstitution: Reconstitute in sterile ddH2O.
• Usage: Reconstitute in ddH₂O to a concentration of 0-0.5 mg/ml. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8°C for one month.; Aliquot and store at -80°C for 12 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37°C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 7706093
• NCBI GeneID: 51284
• NCBI Accession #: NP_057646.1
• NCBI GenBank Nucleotide #: NM_016562.3
• UniProt Accession #: Q9NYK1; Q9NR98; D1CS69
• Molecular Weight: Predicted MW: 21.3 kDa; Accurate MW: 21 kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: toll-like receptor 7
• NCBI Official Synonym Full Names: toll-like receptor 7
• NCBI Official Symbol: TLR7
• NCBI Official Synonym Symbols: TLR7-like
• NCBI Protein Information: toll-like receptor 7; toll-like receptor 7-like
• UniProt Protein Name: Toll-like receptor 7
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR7
• UniProt Entry Name: TLR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR7: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1. Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; endoplasmic reticulum; lysosome; cytoplasm; plasma membrane; integral to membrane; endosome membrane; endosome; receptor complex

Molecular Function: siRNA binding; single-stranded RNA binding; transmembrane receptor activity; double-stranded RNA binding; drug binding

Biological Process: positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-6 production; microglial cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 7 signaling pathway; positive regulation of chemokine production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-8 production; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; I-kappaB phosphorylation; defense response to virus; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process