Angiopoietin-related protein 3 ELISA Kit | ANGPTL3 elisa kit

Human ANGPTL3 PicoKine ELISA Kit

Cat. Num. AAA177542

• Gene Names: ANGPTL3; ANL3; ANG-5; FHBL2; ANGPT5
• Reactivity: Human. There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: Angiopoietin-related protein 3; Human ANGPTL3 PicoKine ELISA Kit; ANG 5; ANG-5; ANG5; Angiopoietin 5; Angiopoietin like 3; Angiopoietin related protein 3; Angiopoietin-5; Angiopoietin-like protein 3; ANGL3_HUMAN; ANGPT5; ANGPTL3; ANL3; FHBL2; OTTHUMP00000010719; UNQ153/PRO179; angiopoietin-like 3; ANGPTL3 elisa kit

• Reactivity: Human. There is no detectable cross-reactivity with other relevant proteins.
• Sequence Length: 460

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Sensitivity: <10pg/ml
• Immunogen: sf21, S17-E460
• Intra-Assay Precision: Sample 1: n=16; Mean(ng/ml): 3.2; Standard deviation: 0.144; CV(%): 4.5 Sample 2: n=16; Mean(ng/ml): 5.8; Standard deviation: 0.273; CV(%): 4.7 Sample 3: n=16; Mean(ng/ml): 10.3; Standard deviation: 0.299; CV(%): 2.9
• Inter-Assay Precision: Sample 1: n=24; Mean(ng/ml): 3.5; Standard deviation: 0.238; CV(%): 6.8 Sample 2: n=24; Mean(ng/ml): 6.2; Standard deviation: 0.459; CV(%): 7.4 Sample 3: n=24; Mean(ng/ml): 12.1; Standard deviation: 0.762; CV(%): 6.3
• Assay Range: 312pg/ml-20,000pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human ANGPTL3 PicoKine ELISA Kit standard curve )

Related Product Information for ANGPTL3 elisa kit

Description: Sandwich ELISA kit of Quantitative Detection for Human ANGPTL3
Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

NCBI and Uniprot Product Information

• NCBI GI #: 7656888
• NCBI GeneID: 27329
• NCBI Accession #: NP_055310.1
• NCBI GenBank Nucleotide #: NM_014495.3
• UniProt Accession #: Q9Y5C1; A0JLS0; B1ALJ0; B2RCW1
• Molecular Weight: 53,637 Da
• NCBI Official Full Name: angiopoietin-related protein 3 preproprotein
• NCBI Official Synonym Full Names: angiopoietin-like 3
• NCBI Official Symbol: ANGPTL3
• NCBI Official Synonym Symbols: ANL3; ANG-5; FHBL2; ANGPT5
• NCBI Protein Information: angiopoietin-related protein 3; angiopoietin 5
• UniProt Protein Name: Angiopoietin-related protein 3
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL3
• UniProt Synonym Gene Names: ANGPT5; ANG-5
• UniProt Entry Name: ANGL3_HUMAN

NCBI Description

This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metablism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. [provided by RefSeq, Feb 2013]

Uniprot Description

ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: extracellular space; cell surface

Molecular Function: enzyme inhibitor activity; integrin binding; phospholipase inhibitor activity; growth factor activity

Biological Process: integrin-mediated signaling pathway; cholesterol metabolic process; glycerol metabolic process; phospholipid catabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; signal transduction; fatty acid metabolic process; sequestering of lipid; positive regulation of angiogenesis; cholesterol homeostasis; acylglycerol homeostasis; phospholipid metabolic process; artery morphogenesis; negative regulation of lipoprotein lipase activity; phospholipid homeostasis; positive regulation of cell migration

Disease: Hypobetalipoproteinemia, Familial, 2