CD166/ALCAM Active Protein | ALCAM active protein

CD166/ALCAM Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122455

• Gene Names: Alcam; BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
• Purity: >98% as determined by SDS-PAGE
• Synonyms: CD166/ALCAM; CD166/ALCAM Protein; Mouse; Recombinant (His Tag); Mouse ALCAM/CD166 Protein (His Tag); AI853494 Protein; BEN Protein; CD166 Protein; DM-GRASP Protein; MuSC Protein; SC1 Protein; activated leukocyte cell adhesion molecule; ALCAM active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys527

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Trp 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Lys 527) of mouse ALCAM (NP_033785.1) precursor was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Immobilized mouse ALCAM-His at 10ug/ml (100uL/well) can bind mouse CD6-Fc. The EC50 of mouse CD6-Fc is 0.08-0.18ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Immobilized mouse ALCAM-His at 10 ug/ml (100 ul/well) can bind mouse CD6-Fc, The EC50 of mouse CD6-Fc is 0.08-0.18 ug/ml.)

Related Product Information for ALCAM active protein

Background: Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

References

Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.Fujiwara H, et al. (2003) Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166). J Clin Endocrinol Metab. 88(7): 3437-43.Jezierska A, et al. (2006) ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy. Med Sci Monit. 12(8): BR263-73.Kahlert C, et al. (2009) Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer. 101(3): 457-64.

NCBI and Uniprot Product Information

• NCBI GI #: 31791059
• NCBI GeneID: 11658
• NCBI Accession #: NP_033785.1
• NCBI GenBank Nucleotide #: NM_009655.2
• UniProt Accession #: Q61490; O70136; Q8CDA5; Q8R2T0
• Molecular Weight: 65,092 Da
• NCBI Official Full Name: CD166 antigen
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: Alcam
• NCBI Official Synonym Symbols: BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: Alcam
• UniProt Entry Name: CD166_MOUSE

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: axon; cell projection; cell soma; external side of plasma membrane; focal adhesion; immunological synapse; integral to membrane; intrinsic to plasma membrane; membrane; plasma membrane; T cell receptor complex

Molecular Function: protein binding

Biological Process: adaptive immune response; axon guidance; cell adhesion; heterophilic cell adhesion; immune system process; motor axon guidance