ALCAM / CD166 recombinant protein

Recombinant Mouse ALCAM / CD166 Protein (His & Fc tag)

Cat. Num. AAA2547221

• Gene Names: Alcam; BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: ALCAM / CD166; Recombinant Mouse ALCAM / CD166 Protein (His & Fc tag); AI853494; BEN; CD166; DM-GRASP; MGC27910; MuSC; SC1; ALCAM.; ALCAM / CD166 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 583

• Application Notes: The recombinant mouse ALCAM/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 748 amino acids and has a predicted molecular mass of 84.2 kDa. Due to glycosylation, in SDS-PAGE under reducing conditions, rmALCAM/Fc migrates with approximately molecular mass of 105-110 kDa & 210 kDa corresponding to the monomer and homodimer.
• Predicted N Terminal: Trp 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ALCAM / CD166 recombinant protein

Background: Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

Description: A DNA sequence encoding the extracellular domain (Met 1-Lys 527) of mouse ALCAM (NP_033785.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 31791059
• NCBI GeneID: 11658
• NCBI Accession #: NP_033785.1
• NCBI GenBank Nucleotide #: NM_009655.3
• UniProt Accession #: Q61490; O70136; Q8CDA5; Q8R2T0
• Molecular Weight: 65,092 Da
• NCBI Official Full Name: CD166 antigen isoform 1
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: Alcam
• NCBI Official Synonym Symbols: BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• UniProt Gene Name: Alcam

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16|16 B5

Cellular Component: axon; cell soma; external side of plasma membrane; focal adhesion; immunological synapse; intrinsic to plasma membrane; T cell receptor complex

Molecular Function: protein binding

Biological Process: axon guidance; heterophilic cell adhesion; motor axon guidance