Mouse Activin A Receptor Type II B (ACVR2B) ELISA Kit | ACVR2B elisa kit

Mouse Activin A Receptor Type II B (ACVR2B) ELISA Kit

Cat. Num. AAA2707564

• Gene Names: Acvr2b; ActRIIB; AI047905; 4930516B21Rik
• Reactivity: Mouse
• Synonyms: Activin A Receptor Type II B (ACVR2B); Mouse Activin A Receptor Type II B (ACVR2B) ELISA Kit; ACVR2-B; ActR-IIB; ACVR2B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Activin A Receptor Type II B (ACVR2B).; No significant cross-reactivity or interference between Activin A Receptor Type II B (ACVR2B) and analogues was observed.
• Sequence Length: 536

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.056ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A Receptor Type II B (ACVR2B) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A Receptor Type II B (ACVR2B) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACVR2B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type II B (ACVR2B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin A Receptor Type II B (ACVR2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin A Receptor Type II B (ACVR2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin A Receptor Type II B (ACVR2B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6680634
• NCBI GeneID: 11481
• NCBI Accession #: NP_031423.1
• NCBI GenBank Nucleotide #: NP_031423.1
• UniProt Accession #: P27040
• NCBI Official Full Name: activin receptor type-2B
• NCBI Official Synonym Full Names: activin receptor IIB
• NCBI Official Symbol: Acvr2b
• NCBI Official Synonym Symbols: ActRIIB; AI047905; 4930516B21Rik
• NCBI Protein Information: activin receptor type-2B
• UniProt Protein Name: Activin receptor type-2B
• Protein Family: Activin receptor
• UniProt Gene Name: Acvr2b
• UniProt Synonym Gene Names: ACTR-IIB
• UniProt Entry Name: AVR2B_MOUSE

Uniprot Description

ACVR2B: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active. The IIB receptor displays a 3- to 4-fold higher affinity for activin A than the II receptor.

Protein type: Membrane protein, integral; EC 2.7.11.30; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type2 subfamily

Cellular Component: protein complex; membrane; cell; cytoplasm; plasma membrane; integral to membrane; receptor complex

Molecular Function: metal ion binding; nucleotide binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; transforming growth factor beta binding; growth factor binding; transferase activity, transferring phosphorus-containing groups; activin binding; protein serine/threonine/tyrosine kinase activity; kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: heart development; regulation of signal transduction; palate development; signal transduction; protein amino acid phosphorylation; post-embryonic development; BMP signaling pathway; anterior/posterior pattern formation; positive regulation of activin receptor signaling pathway; regulation of transcription, DNA-dependent; embryonic foregut morphogenesis; transmembrane receptor protein serine/threonine kinase signaling pathway; lymphangiogenesis; pancreas development; response to glucose stimulus; mesoderm development; kidney development; skeletal development; gastrulation with mouth forming second; positive regulation of bone mineralization; pattern specification process; odontogenesis of dentine-containing teeth; organ growth; positive regulation of osteoblast differentiation; skeletal morphogenesis; insulin secretion; activation of protein kinase activity; blood vessel remodeling; determination of left/right symmetry; phosphorylation; lung development