Rabbit anti-Human, Mouse Acvr2b Polyclonal Antibody | anti-Acvr2b antibody

Acvr2b, ID (Acvr2b, Activin receptor type-2B, Activin receptor type IIB) (AP)

Cat. Num. AAA6271431

• Gene Names: Acvr2b; ActRIIB
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Acvr2b; Polyclonal Antibody; ID (Acvr2b; Activin receptor type-2B; Activin receptor type IIB) (AP); Activin receptor type IIB; anti-Acvr2b antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Activin Receptor Type IIB at ~55kD. Species crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Acvr2b antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Acvr2b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-176 amino acids from the Central region of Mouse Acvr2b.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Acvr2b Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Acvr2b antibody detected the Acvr2b protein (arrow).)

Western Blot (WB)

(Mouse Acvr2b Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Acvr2b antibody detected the Acvr2b protein (arrow).)

Related Product Information for anti-Acvr2b antibody

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.

Product Categories/Family for anti-Acvr2b antibody

Antibodies; Activin

References

Natale, D.R., et al. Dev. Biol. 335(1):120-131(2009). Gamer, L.W., et al. Dev. Dyn. 238(9):2374-2381(2009). Yamamoto, M., et al. J. Cell Biol. 184(2):323-334(2009). Morita, S., et al. PLoS ONE 4 (1), E4212 (2009): Landry, J., et al. PLoS Genet. 4 (10), E1000241 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 6680634
• NCBI GeneID: 11481
• NCBI Accession #: NP_031423.1
• NCBI GenBank Nucleotide #: NM_007397.2
• UniProt Accession #: P27040; Q3KQI1
• Molecular Weight: 60,542 Da
• NCBI Official Full Name: activin receptor type-2B
• NCBI Official Synonym Full Names: activin receptor IIB
• NCBI Official Symbol: Acvr2b
• NCBI Official Synonym Symbols: ActRIIB
• NCBI Protein Information: activin receptor type-2B; ACTR-IIB; activin receptor type IIB
• UniProt Protein Name: Activin receptor type-2B
• Protein Family: Activin receptor
• UniProt Gene Name: Acvr2b
• UniProt Synonym Gene Names: ACTR-IIB
• UniProt Entry Name: AVR2B_MOUSE

Uniprot Description

ACVR2B: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active. The IIB receptor displays a 3- to 4-fold higher affinity for activin A than the II receptor.

Protein type: Membrane protein, integral; EC 2.7.11.30; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type2 subfamily

Cellular Component: protein complex; membrane; cell; cytoplasm; plasma membrane; integral to membrane; receptor complex

Molecular Function: metal ion binding; nucleotide binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; transforming growth factor beta binding; growth factor binding; transferase activity, transferring phosphorus-containing groups; activin binding; protein serine/threonine/tyrosine kinase activity; kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: heart development; regulation of signal transduction; palate development; signal transduction; protein amino acid phosphorylation; post-embryonic development; BMP signaling pathway; anterior/posterior pattern formation; positive regulation of activin receptor signaling pathway; regulation of transcription, DNA-dependent; embryonic foregut morphogenesis; transmembrane receptor protein serine/threonine kinase signaling pathway; lymphangiogenesis; pancreas development; response to glucose stimulus; mesoderm development; kidney development; skeletal development; gastrulation with mouth forming second; positive regulation of bone mineralization; pattern specification process; odontogenesis of dentine-containing teeth; organ growth; positive regulation of osteoblast differentiation; skeletal morphogenesis; insulin secretion; activation of protein kinase activity; blood vessel remodeling; determination of left/right symmetry; phosphorylation; lung development