Western Blot (WB)
(Western blot analysis of SIVA expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse SIVA Polyclonal Antibody | anti-SIVA antibody
SIVA Antibody
Gene Names
SIVA1; SIVA; CD27BP; Siva-1; Siva-2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SIVA; Polyclonal Antibody; SIVA Antibody; Apoptosis regulatory protein Siva; CD27 binding (Siva) protein; CD27-binding protein; CD27BP; Siva 1; Siva 2; SIVA_HUMAN; Siva1; anti-SIVA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SIVA antibody detects endogenous levels of total SIVA
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
175
Applicable Applications for anti-SIVA antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Nucleus. In the nucleus, accumulates in dot-like structures.
Tissue Specificity
Ubiquitous. Mostly expressed in thymus, testis, ovary, prostate, small intestine and spleen and less in colon.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep
Similarity
Pig (92%), Bovine (92%), Horse (92%), Sheep (92%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of SIVA expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of SIVA expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9603881 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603881 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SIVA antibody
Function: Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis.
Subunit Structure: Binds through its N-terminal region to the C-terminus of CD27 and to PXMP2/PMP22. Binds to the C-terminus of TNFRSF18/GITR. Isoform 1 binds to BCL2L1/BCLX isoform Bcl-x(L) but not to BAX. Binds to capsid protein VP2 from coxsackievirus B3. Binding of SIVA1 to CD27 can be blocked by VP2.
Post-translational Modifications: Phosphorylated by ABL2/ARG in response to oxidative stress.
Subunit Structure: Binds through its N-terminal region to the C-terminus of CD27 and to PXMP2/PMP22. Binds to the C-terminus of TNFRSF18/GITR. Isoform 1 binds to BCL2L1/BCLX isoform Bcl-x(L) but not to BAX. Binds to capsid protein VP2 from coxsackievirus B3. Binding of SIVA1 to CD27 can be blocked by VP2.
Post-translational Modifications: Phosphorylated by ABL2/ARG in response to oxidative stress.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 19 kDa
Predicted: 19 kDa
Predicted: 19 kDa
NCBI Official Full Name
apoptosis regulatory protein Siva isoform 1
NCBI Official Synonym Full Names
SIVA1 apoptosis inducing factor
NCBI Official Symbol
SIVA1
NCBI Official Synonym Symbols
SIVA; CD27BP; Siva-1; Siva-2
NCBI Protein Information
apoptosis regulatory protein Siva
UniProt Protein Name
Apoptosis regulatory protein Siva
UniProt Gene Name
SIVA1
UniProt Synonym Gene Names
SIVA; CD27BP
NCBI Description
This gene encodes an E3 ubiquitin ligase that regulates cell cycle progression, cell proliferation and apoptosis. The N-terminus of this protein binds to the cytoplasmic tail of the CD27 antigen, a member of the tumor necrosis factor receptor (TNFR) superfamily. In response to UV radiation-induced DNA damage, this protein has been shown to mediate the ubiquitination of proliferating cell nuclear antigen (PCNA), an important step in translesion DNA synthesis. [provided by RefSeq, Sep 2018]
Uniprot Description
Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis.
Research Articles on SIVA
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Product Notes
The SIVA siva1 (Catalog #AAA9603881) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIVA Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SIVA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SIVA siva1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIVA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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