ACVR2B active protein

ACVR2B Protein, Mouse, Recombinant (His & hFc Tag)

Cat. Num. AAA8122582

• Gene Names: Acvr2b; ActRIIB
• Purity: >97% as determined by SDS-PAGE
• Synonyms: ACVR2B; ACVR2B Protein; Mouse; Recombinant (His & hFc Tag); Mouse ACVR2B Protein (His & Fc Tag); 4930516B21Rik Protein; ActRIIB Protein; activin A receptor; type IIB; ACVR2B active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr134

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser l9
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Thr 134) of mouse ACVR2B (NP_031423.1) precursor was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its ability to bind biotinylated Human INHBA-his in functional ELISA.; 2. Measured by its ability to bind biotinylated mouse INHBA-his in functional ELISA.; 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50ng/mL in the presence of 10ng/mL recombinant Activin A.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.)

Related Product Information for ACVR2B active protein

Background: ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.

References

Kosaki R, et al. (1999) Left-right axis malformations associated with mutations in ACVR2B, the gene for human activin receptor type IIB. Am J Med Genet. 82(1):70-6. Dupont S, et al. (2001) No evidence for linkage or for diabetes-associated mutations in the activin type 2B receptor gene (ACVR2B) in French patients with mature-onset diabetes of the young or type 2 diabetes. Diabetes 50(5):1219-21. Albertson RC, et al. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental dynamics 233(4): 1405-18. Walsh S, et al. (2007) Activin-type II receptor B (ACVR2B) and follistatin haplotype associations with muscle mass and strength in humans. J Appl Physiol. 102(6):2142-8.

NCBI and Uniprot Product Information

• NCBI GI #: 6680634
• NCBI GeneID: 11481
• NCBI Accession #: NP_031423.1
• NCBI GenBank Nucleotide #: NM_007397.2
• UniProt Accession #: P27040; Q3KQI1
• Molecular Weight: 60,542 Da
• NCBI Official Full Name: activin receptor type-2B
• NCBI Official Synonym Full Names: activin receptor IIB
• NCBI Official Symbol: Acvr2b
• NCBI Official Synonym Symbols: ActRIIB
• NCBI Protein Information: activin receptor type-2B; ACTR-IIB; activin receptor type IIB
• UniProt Protein Name: Activin receptor type-2B
• Protein Family: Activin receptor
• UniProt Gene Name: Acvr2b
• UniProt Synonym Gene Names: ACTR-IIB
• UniProt Entry Name: AVR2B_MOUSE

Uniprot Description

ACVR2B: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active. The IIB receptor displays a 3- to 4-fold higher affinity for activin A than the II receptor.

Protein type: Membrane protein, integral; EC 2.7.11.30; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type2 subfamily

Cellular Component: protein complex; membrane; cell; cytoplasm; plasma membrane; integral to membrane; receptor complex

Molecular Function: metal ion binding; nucleotide binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; transforming growth factor beta binding; growth factor binding; transferase activity, transferring phosphorus-containing groups; activin binding; protein serine/threonine/tyrosine kinase activity; kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: heart development; regulation of signal transduction; palate development; signal transduction; protein amino acid phosphorylation; post-embryonic development; BMP signaling pathway; anterior/posterior pattern formation; positive regulation of activin receptor signaling pathway; regulation of transcription, DNA-dependent; embryonic foregut morphogenesis; transmembrane receptor protein serine/threonine kinase signaling pathway; lymphangiogenesis; pancreas development; response to glucose stimulus; mesoderm development; kidney development; skeletal development; gastrulation with mouth forming second; positive regulation of bone mineralization; pattern specification process; odontogenesis of dentine-containing teeth; organ growth; positive regulation of osteoblast differentiation; skeletal morphogenesis; insulin secretion; activation of protein kinase activity; blood vessel remodeling; determination of left/right symmetry; phosphorylation; lung development