ACVR2B recombinant protein

Recombinant Mouse ACVR2B Protein (His & Fc tag)

Cat. Num. AAA2547124

• Gene Names: Acvr2b; ActRIIB; 4930516B21Rik
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ACVR2B; Recombinant Mouse ACVR2B Protein (His & Fc tag); ActRIIB; MGC118477; ACVR2B recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 536

• Application Notes: The recombinant mouse ACVR2B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 364 amino acids and has a predicted molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B/Fc monomer is approximately 60-65 kDa due to glycosylation.
• Predicted N Terminal: Ser l9
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ACVR2B recombinant protein

Background: ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.

Description: A DNA sequence encoding the extracellular domain (Met 1-Thr 134) of mouse ACVR2B (NP_031423.1) precursor was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6680634
• NCBI GeneID: 11481
• NCBI Accession #: NP_031423.1
• NCBI GenBank Nucleotide #: NM_007397.3
• UniProt Accession #: P27040; Q3KQI1
• Molecular Weight: 56,853 Da
• NCBI Official Full Name: activin receptor type-2B
• NCBI Official Synonym Full Names: activin receptor IIB
• NCBI Official Symbol: Acvr2b
• NCBI Official Synonym Symbols: ActRIIB; 4930516B21Rik
• NCBI Protein Information: activin receptor type-2B
• UniProt Protein Name: Activin receptor type-2B
• Protein Family: Activin receptor
• UniProt Gene Name: Acvr2b
• UniProt Synonym Gene Names: ACTR-IIB

Uniprot Description

ACVR2B: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active. The IIB receptor displays a 3- to 4-fold higher affinity for activin A than the II receptor.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type2 subfamily

Chromosomal Location of Human Ortholog: 9|9 F3

Cellular Component: cytoplasm; protein complex; receptor complex

Molecular Function: activin binding; growth factor binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; transforming growth factor beta binding

Biological Process: activation of protein kinase activity; activin receptor signaling pathway; anterior/posterior pattern formation; blood vessel remodeling; BMP signaling pathway; determination of left/right symmetry; embryonic foregut morphogenesis; gastrulation with mouth forming second; heart development; insulin secretion; kidney development; lung development; lymphangiogenesis; mesoderm development; negative regulation of transcription from RNA polymerase II promoter; odontogenesis of dentine-containing teeth; organ growth; palate development; pancreas development; pattern specification process; positive regulation of activin receptor signaling pathway; positive regulation of bone mineralization; positive regulation of osteoblast differentiation; post-embryonic development; regulation of signal transduction; regulation of transcription, DNA-dependent; response to glucose stimulus; signal transduction; skeletal development; skeletal morphogenesis