Mouse Interleukin 3 (IL3) ELISA Kit | IL3 elisa kit

Mouse Interleukin 3 (IL3) ELISA Kit

Cat. Num. AAA454390

• Gene Names: Il3; BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• Reactivity: Mouse
• Synonyms: Interleukin 3 (IL3); Mouse Interleukin 3 (IL3) ELISA Kit; MCGF; MULTI-CSF; MCSF; M-CSF; Multiple; Multilineage-Colony-Stimulating Factor; Hematopoietic Growth Factor; P-Cell Stimulating Factor; Mast-Cell Growth Factor; IL3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL3. No significant cross-reactivity or interference between IL3 and analogues was observed.
• Sequence Length: 166

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 7.4pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL3 in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31982791
• NCBI GeneID: 16187
• NCBI Accession #: NP_034686.2
• NCBI GenBank Nucleotide #: NM_010556.4
• UniProt Accession #: P01586
• Molecular Weight: 18,540 Da
• NCBI Official Full Name: interleukin-3
• NCBI Official Synonym Full Names: interleukin 3
• NCBI Official Symbol: Il3
• NCBI Official Synonym Symbols: BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• NCBI Protein Information: interleukin-3
• UniProt Protein Name: Interleukin-3
• Protein Family: Interleukin
• UniProt Gene Name: Il3
• UniProt Synonym Gene Names: Csfmu; Il-3; IL-3; MCGF

Uniprot Description

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.