anti-Any Mouse Anti-Methylglyoxal Monoclonal Antibody
Mouse Anti-Methylglyoxal Monoclonal Antibody
Reactivity
Any
Applications
Immunoblot, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
Mouse Anti-Methylglyoxal Monoclonal Antibody; anti-Mouse Anti-Methylglyoxal Monoclonal Antibody antibody
Host
Mouse
Reactivity
Any
Clone Number
3D11
Specificity
MG-modified proteins, lipids and nucleic acids (MG-H1(methyl-glyoxal-hydro-imidazolone) based on HPLC and GC-MS). 3D11 does not react with CML, CEL, or other AGE epitopes.
Purity/Purification
Affinity Purified
Form/Format
100 ug of affinity purified antibody at 0.85 mg/mL in PBS containing 0.2% 5-bromo-5-nitro-1,3-dioxane
Applicable Applications for anti-Mouse Anti-Methylglyoxal Monoclonal Antibody antibody
Immunoblot (IB), Immunohistochemistry (IHC)
Application Notes
Immunoblot (1:1000 to 1:4000)
Immunohistochemistry (1:20 to 1:60)
Immunohistochemistry (1:20 to 1:60)
Immunogen
MG-modified ovalbumin
Conjugation
Unconjugated
Quantity
100 ug of affinity purified antibody at 0.6 mg/mL in PBS containing 0.2% 5-bromo-5-nitro-1,3-dioxane
Preparation and Storage
-20 degree C. Shelf Life: 1 year from date of receipt under proper storage conditions; aliquot to avoid multiple freeze thaw cycles
Related Product Information for anti-Mouse Anti-Methylglyoxal Monoclonal Antibody antibody
• Affinity purified mouse monoclonal
The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. Tissue levels of AGE increase with age and the formation of AGEs is predominantly endogenous, though these products can also be derived from exogenous sources such as food and tobacco smoke. AGE modification of proteins can contribute to the pathophysiology of aging and long-term complications of diabetes, atherosclerosis and renal failure. AGEs also interact with a variety of cell-surface AGE-binding receptors (RAGE), leading either to their endocytosis and degradation or to cellular activation and pro-oxidant or pro-inflammatory events.
Several AGE structures have been reported, such as Nepsilon-(carboxymethyl) lysine (CML), Nepsilon-(carboxyethyl) lysine (CEL), pentosidine, and Methylglyoxal (MG) derivatives. MG is formed through non-oxidative mechanisms from triose phosphates during anaerobic glycolysis and it can modify amino acids, nucleic acids, and proteins. MG reacts with arginine, lysine and cysteine residues of proteins to form AGEs. MG is involved in various pathological processes. For example, MG derivatives are found elevated in diabetes.
The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. Tissue levels of AGE increase with age and the formation of AGEs is predominantly endogenous, though these products can also be derived from exogenous sources such as food and tobacco smoke. AGE modification of proteins can contribute to the pathophysiology of aging and long-term complications of diabetes, atherosclerosis and renal failure. AGEs also interact with a variety of cell-surface AGE-binding receptors (RAGE), leading either to their endocytosis and degradation or to cellular activation and pro-oxidant or pro-inflammatory events.
Several AGE structures have been reported, such as Nepsilon-(carboxymethyl) lysine (CML), Nepsilon-(carboxyethyl) lysine (CEL), pentosidine, and Methylglyoxal (MG) derivatives. MG is formed through non-oxidative mechanisms from triose phosphates during anaerobic glycolysis and it can modify amino acids, nucleic acids, and proteins. MG reacts with arginine, lysine and cysteine residues of proteins to form AGEs. MG is involved in various pathological processes. For example, MG derivatives are found elevated in diabetes.
Product Categories/Family for anti-Mouse Anti-Methylglyoxal Monoclonal Antibody antibody
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Product Notes
The Mouse Anti-Methylglyoxal Monoclonal Antibody (Catalog #AAA168596) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Methylglyoxal Monoclonal Antibody reacts with Any and may cross-react with other species as described in the data sheet. AAA Biotech's Mouse Anti-Methylglyoxal Monoclonal Antibody can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), Immunohistochemistry (IHC). Immunoblot (1:1000 to 1:4000) Immunohistochemistry (1:20 to 1:60). Researchers should empirically determine the suitability of the Mouse Anti-Methylglyoxal Monoclonal Antibody for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse Anti-Methylglyoxal Monoclonal Antibody, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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