Western Blot (WB)
(Fig. Western blot analysis of 2ug His fusion protein with HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) in 1:2000 (lane A) and 1:5000 (lane B) dilutions.)
Mouse His Tag Monoclonal Antibody | anti-His antibody
HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)
Applications
Western Blot, Immunoprecipitation
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Synonyms
His Tag; Monoclonal Antibody; HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3); His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag; anti-His antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Form/Format
Liquid in PBS, pH7.4 and 50% Glycerol.
Applicable Applications for anti-His antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:3000-1:10000
Tag
His
Conjugation
HRP
Immunogen
Synthetic Peptide
Preparation and Storage
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Western Blot (WB)
(Fig. Western blot analysis of 2ug His fusion protein with HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) in 1:2000 (lane A) and 1:5000 (lane B) dilutions.)
Western Blot (WB)
(Fig. Western blot analysis of 2ug His fusion protein with HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) in 1:2000 (lane A) and 1:5000 (lane B) dilutions.)
Related Product Information for anti-His antibody
Description: Featured HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3), specially designed for your taged protein quantification and localization.
Background: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Background: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Product Categories/Family for anti-His antibody
Similar Products
Product Notes
The His (Catalog #AAA9719142) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:3000-1:10000. Researchers should empirically determine the suitability of the His for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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